Concentration of dissolved inorganic carbon (DIC), carbon and nitrogen concentrations, C:N ratios and del 13C isotope value for lakes and rivers on North Slope from Brooks Range to Prudhoe Bay, Arctic LTER 1988 to 2005

Abstract: 

Composite file describing plant, animal, water, and sediment samples collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number specific to the file, a number that relates the samples to other samples collected on the same date and time (sortchem), site, date, time, and depth. Samples are identified by type, category, and a short description. Data include isotope values, carbon and nitrogen concentrations, and C:N ratios of samples.

Project Keywords: 

Data set ID: 

321

EML revision ID: 

8

Short name: 

1988-2005_Isotopes_Kling

DOI: 

10.6073/pasta/6341694e9d7155735d17da7001014e18
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Thursday, June 25, 1998 to Wednesday, August 24, 2005

Publication Date: 

Wednesday, December 12, 2007

Methods: 

For water sampling protocol, see the Land-Water protocol accessible from the Land-Water main page on the Arctic LTER web page or at http://www-personal.umich.edu/~gwk and clicking on the protocol link at the bottom of the page.

Specific methodologies are described in the protocol book listed above and a general description is provided below. See also,
Kling, G. W., B. Fry, and W. J. O'Brien. 1992. Stable isotopes and planktonic trophic structure in arctic lakes. Ecology 73:561-566.

All organic matter samples (sediments, algae, animals, etc.) were dried at temperatures less than 45 degC to prevent volatization.

Particulate organic carbon (POM) samples were collected from the water column by filtering as much sample water through a 25 or 45 mm Whatman GF/F filter as possible. Attached algae were collected from surfaces (sediments, rocks, plants), and aquatic macrophytes were collected using the most recent growth tissues.

Zooplankton species were identified and individuals were separated by hand, then grouped by species for isotopic determination. Other benthic or pelagic organisms were separated by hand to species or to the nearest taxonomic group. Species with carbonate shells were bathed in weak acid to remove inorganic C contamination before drying and analysis.

Samples for the determination of 13C-DIC were collected using serum vials following the DIC collection technique described in detail in the Kling laboratory protocol (Old DIC method). Samples are collected at depths representative of the epilimnion, metalimnion, and hypolimnion (if present) or from the epilimnion in shallow lakes. The depths were commonly 1 m, 3 - 5 m, and 8 - 9 m for lake E5 and 1 m for lake E6. The sampling depths are determined in the field and are based upon the depth profile of temperature and chlorophyll estimated by fluorescence using a Hydrolab multisensor datalogger. Samples were collected using a Van Dorn water sampler lowered to the specific depths and subsampled using large Nalgene bottles. A bubble-free subsample (no exposure to the atmosphere) was then over-filled (for flushing to remove bubbles) into 30 or 60 mL serum vials. A supersaturated solution of mercuric chloride was added (0.1 mL solution per 10 mL sample) to the sample to stop biological activity in the bottle and the vial was capped with an aluminum cap lined with a teflon septa.

Organic and sediment samples were analyzed mainly at the Marine Biological Laboratory (MBL) Stable Isotope Laboratory for 13C and 15N.(1)

13C-DIC samples were analyzed by the Stable Isotope Laboratory at the University of Michigan on a Finnigan MAT Delta S. (2)

(1) Marine Biological Laboratory (MBL) Stable Isotope Laboratory: http://ecosystems.mbl.edu/SILAB/default.html
The Ecosystems Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543 U.S.A.
(2) University of Michigan Stable Isotope Laboratory: http://www-personal.umich.edu/%7Eloraw/

http://www-personal.umich.edu/~gwk

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

Use of the data requires acceptance of the data use policy --> Arctic LTER Data Use Policy

To cite this data set see the citation example on the LTER Network Data Portal page for this data set.

Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
Isotope ID
  • Label: Isotope ID
  • Definition: A unique number assigned to each sample within the Isotope file. This number is unrelated to the sortchem.
  • Type: Nominal
  • Missing values: None specified
SortChem
  • Label: SortChem
  • Definition: A unique number assigned to each sample collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600.
  • Type: Nominal
  • Missing values: None specified
Site
  • Label: Site
  • Definition: Site Name. If a soil site, the site is described in the name by general location then specific feature (watertrack,etc) then specific point (1,2,3,...). If a lake or streams site, the lake or stream name (as it is commonly known) is given. Exceptions in naming procedure are provided in the Code Information and in AK-LTER_Site_Info.xls.
  • Type: Nominal
  • Missing values: None specified
Date
  • Label: Date
  • Definition: Sampling Date
  • Type: Date/time
  • Date format: dd-mmm-yyyy
  • Missing values:
    • . = Missing or Not Measured
Time_hr_dst
  • Label: Time_hr_dst
  • Definition: Sampling Time in Alaska Daylight Savings Time (1 hour ahead of Alaska Standard Time: if 13:00 DST than 12:00 AST)
  • Type: Date/time
  • Date format: hh:mm
  • Missing values:
    • . = Missing or Not Measured
Depth_m
  • Label: Depth_m
  • Definition: Depth from surface of lake, stream, or soil. In meters or lake zone.
  • Type: Nominal
  • Missing values: None specified
Type
  • Label: Type
  • Definition: General category describing the sample type.
  • Type: Nominal
  • Missing values: None specified
Category
  • Label: Category
  • Definition: Subcategory of "Type" category which further describes the sample.
  • Type: Nominal
  • Missing values: None specified
Description
  • Label: Description
  • Definition: Description of sample, often giving taxonometric or filter information. This column contains the original information used to describe a sample.
  • Type: Nominal
  • Missing values: None specified
del15N_‰
  • Label: del15N_‰
  • Definition: Delta value of 15N with respect to air (standard)
  • Type: Physical quantity
  • Unit: partPerThousand
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
N_umol_L
  • Label: N_umol_L
  • Definition: Nitrogen concentration
  • Type: Physical quantity
  • Unit: micromolePerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
N_%_Mass
  • Label: N_%_Mass
  • Definition: Percent nitrogen content by mass.
  • Type: Physical quantity
  • Unit: percent
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
del13C_‰
  • Label: del13C_‰
  • Definition: Delta value of 13C with respect to VPDB (Vienna Peedee Belemnite standard)
  • Type: Physical quantity
  • Unit: partPerThousand
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
C_umol_L
  • Label: C_umol_L
  • Definition: Concentration of carbon in sample
  • Type: Physical quantity
  • Unit: micromolePerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
C_%_Mass
  • Label: C_%_Mass
  • Definition: Percent carbon content by mass
  • Type: Physical quantity
  • Unit: percent
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Ratio_C_to_N
  • Label: Ratio_C_to_N
  • Definition: Ratio of carbon to nitrogen by mass
  • Type: Physical quantity
  • Unit: dimensionless
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured