Number of bacteria in the water column of lakes sampled near Toolik Lake LTER Alaska, throughout summer season, 1992-2000.

Abstract: 

Number of bacteria (using of DAPI for identifying and counting) in Toolik Lake water column and other lakes sample throught the summer from 1992-2000. There is no data for 2006.

Project Keywords: 

Data set ID: 

10301

EML revision ID: 

7

Short name: 

1992-2000hobact

DOI: 

10.6073/pasta/0a142f2a8cd8fb5e7bf7133e1a938b2b
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Friday, May 1, 1992 to Friday, September 1, 2000

Publication Date: 

Thursday, December 12, 2013

Methods: 

For years 1992 -1994

2 ml of gluteraldehyde-preserved lake water was stained with 200 ul 100ug/ml
4'6 diamidino-2-phenylindole (DAPI), final concentration 10 ug/ml, in a 15 ml
Millipore filtration apparatus. After 5 minutes, the sample was vacuum filtered
through a 0.22 um pore size black Nuclepore 25mm filter. The filter was mounted
on a microscope slide using Cargille B immersion oil. Ten fields were counted
using a Zeiss epifluoresencce microscope.

Cell count= (x/field)(2.04 x 10^4 field/ml) = #/ml

For years 1995-2000

After transport to the laboratory at Woods Hole, MA, 2 ml of glutaraldehyde- preserved lake water from each depth was mixed with 2 ml 0.2 um filtered phosphate buffered saline to reduce humic interference and 0.1% Triton X-100 or Nonidet P-40 to improve cell permeability and stored overnight at 4*C. The sample was stained with 200 ul 100ug/ml 4'6 diamidino-2-phenylindole (DAPI), final concentration 10 ug/ml. After 5 minutes, the sample was vacuum filtered through a 0.22 um pore size black Nuclepore 25mm filter in a 15 ml Millipore filtration apparatus. The filter was mounted on a microscope slide using Cargille A immersion oil. Ten fields were counted using a Zeiss epifluoresencce microscope. Reference Citations: Hobbie, J.E., T.L. Corliss and B.J. Peterson, Seasonal patterns of bacterial abundance in an Arctic lake. 1983. Arctic and Alpine Research 15:252-259. Porter, K.G. and Y.S.Feig The use of DAPI for identifying and counting aquatic microflora. 1980. Limnology and Oceanography 25:943-948

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

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File 1992-2000hobact.csv

Number of bacteria in... File Details

File 1992-2000hobact.xlsx

Excel file with... File Details

Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
Site Code
  • Label: Site Code
  • Definition: Site code (LTER code)
  • Type: Nominal
  • Missing values: None specified
Site
  • Label: Site
  • Definition: Site of measurement
  • Type: Nominal
  • Missing values: None specified
Station
  • Label: Station
  • Definition: Station
  • Type: Nominal
  • Missing values: None specified
Depth
  • Label: Depth
  • Definition: Depth in meters
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Date
  • Label: Date
  • Definition: Date
  • Type: Date/time
  • Date format: dd-mmm-yyyy
  • Missing values:
    • . = Missing or Not Measured
Count
  • Label: Count
  • Definition: cell count= # cells x 10^6/ml
  • Type: Physical quantity
  • Unit: number
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Comments
  • Label: Comments
  • Definition: Notes about data
  • Type: Nominal
  • Missing values: None specified