Yearly file containing information on bacterial productivity. Samples were collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number (sortchem), site, date, time and depth.
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For the bacterial production protocol, see the Land-Water protocol accessible from the Landscape Interaactionr mainpage on the Arctic LTER web page or at http://www-personal.umich.edu/~gwk and clicking on the protocol link at the bottom of the page. Bacterial production is determined by the incorporation of 13C labeled Leucine (Simon and Azam 1989). Water samples are vacuum filtered through 0.22 um Millapore GS cellulose nitrate filters. Samples are extracted with 5mL of 5% TCA for 5 minutes, rinsed with additional TCA, and air dried in mini-scintillation vials. Following the addition of 1 mL cellusolve, filters are left to dissolve overnight. Scinti-safe scintillation cocktail (4 mL) is then added to each vial and samples are counted at Toolik Field Station on a liquid scintillation counter (Packard Tri-Carb 2100TR) . Calculations of bacterial productivity assume that the Internal Dilution (ID) of leucine is 1, but studies show it may range from 1-2 (Kirchman 1993). Some published data from these LTER files have used an ID value different than 1; in those studies, the ID value used is stated in the article. 1. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series 51:201-213. 2. Kirchmann, D.L. 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. Pages 509-512 in P.F. Kemp, B.F. Sherr, E.B. Sherr, and J.J. Cole, editors. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Florida, USA. http://arc.lternet.edu/landwater/lw_protocols
Entered metadata into new metadata form. Version 3: Data URL was changed to point to the LTERNET Data Access server Version 10: Updated metadata form to newer version (without site sheet). Fixed discrepency with header variable names. CH 1Feb2013 Updated metadata form to newer version (with site sheet) CH March 2013. Time format updated, new urls and eml files for web created JD-Nov2013 Version 11: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14 Version 12: Changed Distrubution URL since the LTER network DAS system is being discontinued. JimL 9Apr2015 Updated data file from Kling - Using only Toolik Main, Toolik Inlet and TW Weir. Mar 2016 JimL
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