Above ground plant and below ground stem biomass was measured in the Arctic LTER acidic tussock tundra experimental plots. Treatments included control, nitrogen plus phosphorus amended plots for either 6 or 13 years and vole exclosure plots with or without amends of nitrogen and phosphorus.
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The LTER acidic tussock site was set up in 1989. The experimental design is four blocks of 5 x 20 meters plots with randomly assigned treatments within each block. Treatments include control (CT), amended with 10 g per square meter of Nitrogen and 5 g per square meter of phosphorus (NP), simple warming with plastic covered greenhouses (GH), simple warming amended with 10 and 5 g per square meter of nitrogen and phosphorus (GHNP) and 50% shade house with and without 10 and 5 g per square meter of nitrogen and phosphorus added (SH and SHNP).
Exclosure plots were setup in July 1996 on extra 5 x 20 meters plots within the four-block design of the 1989 LTER acidic tussock experimental plots. On each plot a 5x10 meter section was fenced with large mesh (4-inch square mesh) and within this fence a 5x5-meter plot was fenced with a small mesh (1/2-inch square mesh). In each block two fenced plots were setup: a plot with no fertilizer and a plot with annual fertilization (NP) treatments of 10 g/m2 Nitrogen (as NH4NO3) and 5 g/m2 Phosphorous (as triple superphosphate).
In this biomass harvest only the control, 1989 NP (NP13), GH, GHNP, control small mesh (EX), NP unfenced (NP6), and NP small mesh (EXNP) treatments were sampled.
Calculations: Units; Tissue mass data are expressed in g/quadrat (400 cm2). Multiply by 25 to get g/m2.
Reference Citations: Shaver, G. R. and F. S. Chapin, III. 1991. Production/biomass relationships and element cycling in contrasting arctic vegetation types. Ecological Monographs 61:1-31.
Biomass quadrats, size 20x20 cm, were taken from Toolik tussock site. Five quadrats were taken from each of four blocks. All aboveground biomass was clipped in 20x20 cm quadrats. Five quadrats were randomly located along line transects in each of four replicate blocks in each treatment. Aboveground biomass is considered "within" the quadrat if it is associated with a meristem that is within the quadrat. Quadrats were sorted within 24 hours into species and then into tissue type. Depending on the harvest, tissue types can be broad categories, i.e. above and below, or more detailed, i.e. inflorescences, new growth, old growth, etc. Leaf area was measured with a LiCor LI-3000A area meter.
The samples were dried at 60 degrees C in a drying oven and after several days weighed to nearest milligram. Samples are then returned to Woods Hole for nutrient analysis. Details are given in Shaver and Chapin (Ecological Monographs, 61(1), 1991, pg. 1-31).
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