Percent C, Percent N and C:N ratio for leaf samples from ITEX flux survey plots for 2003-2004, Toolik Alaska.

Abstract: 

Foliar carbon and nitrogen concentrations of the dominant species from within the ITEX flux survey plots 2003-2004. Plots were located in the Toolik Lake LTER moist acidic tussock experiment plots in Alaska; at Imnavait Creek, Alaska.

Project Keywords: 

Data set ID: 

10005

EML revision ID: 

8

Short name: 

2003-2004gsfluxleafN
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Friday, August 8, 2003 to Saturday, August 14, 2004

Publication Date: 

Wednesday, December 12, 2007

Methods: 

EXPERIMENTAL DESIGN AND METHODS: (B.Kaye)

SAMPLE METHODS:
Toolik 2003
Samples of top 3 most abundant species in each plot were collected near flux plots on August 8th. C-N leaves were collected 2-2.5 weeks after last round of fluxing was completed.

C-N analysis was done on these leaves by Lorna Street at the University of Edinburgh in the fall of 2003.

Toolik/Imnavait 2004
Leaf samples were collected of the predominate 2-5 species in each flux plot. Leaf samples from the plot (or near the plot) were collected within a few days of the date that the plot was fluxed. Sometimes one group of leaves was collected to represent a group of plots when plots were from the same treatment group and vegetation type and were close together.

These leaves were ground in 2005/2006 at MBL and analyzed for C-N content by Brooke Kaye.

2003 C-N analysis
Leaf samples for C:N analysis from 2003 were processed in 2003 by Lorna Street at the University of Edinburgh, UK. Samples were ground and analysed for carbon and nitrogen concentration using CE-440 Elemental Analyser (North Chelmsford MA, USA).

2005 C-N analysis
Samples from 2004-2005 were ground and analyzed between October 2005 and April 2006 by Brooke Kaye at the Ecosystems Center in Woods Hole, MA.

Samples were briefly re-dried at 60oC (24 hours) and put in a dessicator. Smaller samples (less than ~0.3 grams, depending on leaf size) were ground for approximately one minute in the Wig-L-Bug in the grinding room at the Marine Biological Lab. Larger samples (larger than ~0.3 grams, depending on leaf size) were ground in the Retsch MM200 double grinder for approximately three minutes, or until samples were finely powdered with no remaining visible plant fibers. If leaves still had petioles attached I removed the petioles with scissors and discarded them before grinding the leaf sample. All ground samples were stored in plastic 20ml scint vials until C-N analysis could be done.

For C-N analysis, tins were packed with 3-4 milligrams of powdered leaves. One tin was packed for every sample type. Every 10th sample had a replicate sample packed from it in order to check the reproducibility of values and the uniformity of the ground sample, this is indicated by an R in the column "rep?"

After prep samples were run, leaves were analyzed in auto-run mode (non-filter type samples). After every 10 samples a blank tin and a K-factor (standard - Acetinilide) were run to check accuracy of values. Samples were run on a Perkins Elmer CHNS/O Series II analyzer, owned by the Ecosystems Center and maintained by Don Burnette in 2005/2006.

COMMENTS:

FOR MORE INFORMATION CONTACT: Gus Shaver, The Ecosystems Center, Woods Hole, MA, 02543, USA

REFERENCE CITATIONS: van Wijk M.T., Williams M. and Shaver G (2005) Tight coupling between leaf area index and foliage N content in arctic plant communities. Oecologia 142:421-427

OTHER DATA FILES TO REFERENCE:
2003-2009gsflux.dat. CO2 and H2O flux data.
2004-2009gscoversp.dat. Percent cover of flux plots by species.
2004-2009gscoverft.dat. Percent cover of flux plots by functional type.
2003-2009gsGPSandveg.dat. GPS locations and description of vegetation for flux plots.
2003-2009gscurveparameters.dat Best fit parameters describing the light response curves measured on each plot.
2003-2009gssoiltemp.dat. Soil temperature and volumetric water content for flux plots.
2003gspointframe.dat. Point intercept sampling of vegetation within flux plots 2003
2003-2009gsharvest.dat. Leaf area, biomass and CHN analysis by species for each harvest.
2003-2009gsharvestLAI-N.dat. Total LAI, Foliar N and NDVI for 20cm x 20cm harvests.

FORMAT OF DATA FILE: ASCII

See Methods.

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

Use of the data requires acceptance of the data use policy --> Arctic LTER Data Use Policy

To cite this data set see the citation example on the LTER Network Data Portal page for this data set.

File 2003-2004gsfluxleafN.csv

Percent C, Percent N... File Details

Office spreadsheet icon 2003-2004gsfluxleafN.xls

Excel file with... File Details

Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
YEAR
  • Label: YEAR
  • Definition: sample year
  • Type: Date/time
  • Date format: YYYY
  • Missing values: None specified
DATE
  • Label: DATE
  • Definition: sample date
  • Type: Date/time
  • Date format: dd-mmm-yy
  • Missing values: None specified
SITE
  • Label: SITE
  • Definition: Toolik
  • Type: Nominal
  • Missing values: None specified
GROUP
  • Label: GROUP
  • Definition: Toolik Lake LTER, or Imnavait Creek
  • Type: Nominal
  • Missing values: None specified
TREATMENT
  • Label: TREATMENT
  • Definition: Control or Fertilised
  • Type: Nominal
  • Missing values: None specified
ASSOCIATED FLUX PLOT ID
  • Label: ASSOCIATED FLUX PLOT ID
  • Definition: lists which flux plots C-N data should be applied to
  • Type: Nominal
  • Missing values: None specified
VEGETATION TYPE
  • Label: VEGETATION TYPE
  • Definition: vegetation type identified by dominate species or general characteristics
  • Type: Nominal
  • Missing values: None specified
SPECIES
  • Label: SPECIES
  • Definition: code for latin name of species consisting of the first three letters of genus and first three letters of species
  • Type: Nominal
  • Missing values: None specified
FUNCTIONAL TYPE
  • Label: FUNCTIONAL TYPE
  • Definition: functional type to which the species belongs
  • Type: Nominal
  • Missing values: None specified
RUN DATE
  • Label: RUN DATE
  • Definition: date of CHN analysis
  • Type: Date/time
  • Date format: dd-mmm-yy
  • Missing values: None specified
%C
  • Label: %C
  • Definition: percent carbon by weight
  • Type: Physical quantity
  • Unit: percent
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
%N
  • Label: %N
  • Definition: percent nitrogen by weight
  • Type: Physical quantity
  • Unit: percent
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
C:N RATIO
  • Label: C:N RATIO
  • Definition: foilar carbon per unit foilar N
  • Type: Physical quantity
  • Unit: number
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values: None specified
rep?
  • Label: rep?
  • Definition: indicates if the entry is a replicated CHN run
  • Type: Nominal
  • Missing values: None specified
Comments
  • Label: Comments
  • Definition: Comments
  • Type: Nominal
  • Missing values: None specified