Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 2003, Arctic LTER, Toolik Research Station, Alaska.

Abstract: 

Yearly file containing information on bacterial productivity. Samples were collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number (sortchem), site, date, time and depth.

Project Keywords: 

Data set ID: 

987

EML revision ID: 

11

Short name: 

2003_bacprods_Kling

DOI: 

10.6073/pasta/e3e51d7a3d60aab985d4807228d65430
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Wednesday, June 18, 2003 to Saturday, August 16, 2003

Publication Date: 

Thursday, January 1, 2004

Methods: 

For the bacterial production protocol, see the ARC LTER Land-Water group protocol accessible from the Land-Water mainpage on the Arctic LTER web page or at http://www-personal.umich.edu/~gwk and clicking on the protocol link at the bottom of the page. Bacterial production is determined by the incorporation of 14C labeled Leucine (Simon and Azam 1989) in triplicate water samples incubated in the dark at in situ temperature for 1-3 hours (typically 2 hrs). Water samples are vacuum filtered through 0.22 um Millapore GS cellulose nitrate filters. Samples are extracted with 5mL of 5% TCA for 5 minutes, rinsed with additional TCA, and air dried in mini-scintillation vials. Following the addition of 1 mL cellosolve (ethylene glycol monoethyl ether), filters are left to dissolve overnight. Scinti-safe scintillation cocktail (4 mL) is then added to each vial and samples are counted at Toolik Field Station on a liquid scintillation counter (Packard Tri-Carb 2100TR) .Calculations of bacterial productivity assume that the Internal Dilution (ID) of leucine is 1, but studies show it may range from 1-2 (Kirchman 1993). Some published data from these LTER files have used an ID value different than 1; in those studies, the ID value used is stated in the article.1. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series 51:201-213.2. Kirchman, D.L. 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. Pages 509-512 in P.F. Kemp, B.F. Sherr, E.B. Sherr, and J.J. Cole, editors. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Florida, USA. http://arc-lter.ecosystems.mbl.edu/landwater/lw_protocols

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

Use of the data requires acceptance of the data use policy --> Arctic LTER Data Use Policy

To cite this data set see the citation example on the LTER Network Data Portal page for this data set.

File 2003_bacprods_Kling.csv

Bacterial Production... File Details

File 2003_bacprods_Kling.xlsx

Excel file with... File Details

Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
SortChem
  • Label: SortChem
  • Definition: A unique number assigned to each sample collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600.
  • Type: Nominal
  • Missing values: None specified
Site
  • Label: Site
  • Definition: Site Name. If a soil site, the site is described in the name by general location then specific feature (e.g., watertrack) then specific sampling point (1,2,3,...). Lake and stream sites use common names (e.g., Toolik) or LTER number codes. Exceptions in naming procedure are provided in the Code Information and in the AK-LTER_Site_Info.xls file.
  • Type: Nominal
  • Missing values: None specified
Date
  • Label: Date
  • Definition: Sampling Date
  • Type: Date/time
  • Date format: dd-mmm-yy
  • Missing values:
    • . = Missing or Not Measured
Time_hr_dst
  • Label: Time_hr_dst
  • Definition: Sampling Time in Alaska Daylight Savings Time (DST is 1 hour ahead of Alaska Standard Time: if 13:00 DST than 12:00 AST)
  • Type: Date/time
  • Date format: hh:mm
  • Missing values:
    • . = Missing or Not Measured
Depth_m
  • Label: Depth_m
  • Definition: Depth from surface of lake, stream, or soil
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Bac_prod_ug C_L_day
  • Label: Bac_prod_ug C_L_day
  • Definition: Bacterial Productivity, ug Carbon per L per day
  • Type: Physical quantity
  • Unit: microgramPerLiterPerDay
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured