Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 2005, Arctic LTER, Toolik Research Station, Alaska.

Abstract: 

Yearly file containing information on bacterial productivity. Samples were collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number (sortchem), site, date, time and depth.

Project Keywords: 

Data set ID: 

989

EML revision ID: 

10
Published on EDI/LTER Data Portal

Citation Suggestion: 

Kling, G. 2006. Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 2005, Arctic LTER, Toolik Research Station, Alaska. Environmental Data Initiative. http://dx.doi.org/10.6073/pasta/1245c6b213b06c35210c8692719f9210
People
Dates

Date Range: 

Tuesday, June 21, 2005 to Thursday, August 18, 2005

Publication Date: 

Sunday, January 1, 2006

Methods: 

For the bacterial production protocol, see the ARC LTER Land-Water group protocol accessible from the Land-Water mainpage on the Arctic LTER web page or at http://www-personal.umich.edu/~gwk and clicking on the protocol link at the bottom of the page. Bacterial production is determined by the incorporation of 14C labeled Leucine (Simon and Azam 1989) in triplicate water samples incubated in the dark at in situ temperature for 1-3 hours (typically 2 hrs). Water samples are vacuum filtered through 0.22 um Millapore GS cellulose nitrate filters. Samples are extracted with 5mL of 5% TCA for 5 minutes, rinsed with additional TCA, and air dried in mini-scintillation vials. Following the addition of 1 mL cellosolve (ethylene glycol monoethyl ether), filters are left to dissolve overnight. Scinti-safe scintillation cocktail (4 mL) is then added to each vial and samples are counted at Toolik Field Station on a liquid scintillation counter (Packard Tri-Carb 2100TR) .Calculations of bacterial productivity assume that the Internal Dilution (ID) of leucine is 1, but studies show it may range from 1-2 (Kirchman 1993). Some published data from these LTER files have used an ID value different than 1; in those studies, the ID value used is stated in the article.1. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series 51:201-213.2. Kirchman, D.L. 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. Pages 509-512 in P.F. Kemp, B.F. Sherr, E.B. Sherr, and J.J. Cole, editors. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Florida, USA. http://arc-lter.ecosystems.mbl.edu/landwater/lw_protocols

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

Use of the data requires acceptance of the data use policy --> Arctic LTER Data Use Policy

To cite this data set see the citation example on the LTER Network Data Portal page for this data set.

File 2005_bacprods_Kling.csv

Bacterial Production... File Details

File 2005_bacprods_Kling.xlsx

Excel file with... File Details