Belowground foodweb biomass from moist acidic tundra and dry heath tundra nutrient addition and herbivore exclusion plots (since 1996) sampled Summer 2006

Abstract: 

Biomass of belowground community groups (bacteria, fungi, protozoa, nematodes, rotifers, tardigrades) determined for organic soils in moist acidic tundra and dry heath tundra.

Project Keywords: 

Data set ID: 

10342

EML revision ID: 

4

Short name: 

2006_MAT_DHT_96NPGraze_FWEB_JCM

DOI: 

10.6073/pasta/928670b8cb8d
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Tuesday, June 6, 2006 to Tuesday, July 25, 2006

Publication Date: 

Friday, December 12, 2008

Methods: 

On 6 June and 25 July 2006, organic horizon soil samples (10x10x15cm in MAT, 10x10x3cm in DHT) were collected from control and N+P treatments from Moist Acidic Tundra and Dry Heath Tundra grazing plots established in 1996. We collected two subsamples from both tussock and inter-tussock areas in each MAT plot, and two subsamples per Dry Heath plot. We determined percent moisture for all soils and quantified the biomass of members of the belowground community.
Direct count slides for microbial biomass estimation were made using the methods of Bloem (1995) as adapted by Frey et al. (1999). 10ml of a soil solution diluted 1:100 (5g initial soil mass) was added to each of 5 6mm diameter wells on a slide. Bacteria slides were stained with DTAF (5-(4, 6 dichlorotriazin-2-yl) aminofluorescein) and Fungi slides were stained with calcifluor M2R fluorescence brightener. Direct counts were made using epifluoresence microscopy. Bacteria were counted, 10 images per well, 2 wells per sample. Fungal hyphal length (Lodge and Ingham 1991) was estimated using the formula of Newman (1966): R=πNA/2H where N=number of hyphae crossing transect line, A=area of well, H=total length of transects. 2 wells, each with 10 images, 3 transects per image were used.
Protozoa densities were estimated from 10 g soil samples using Most Probable Number MPN (Darbyshire et al. 1974; Ingham 1994). 10 g soil was serially diluted 10-1-10-6. Using a 24-well tissue culture plate, four replicate wells of each of the six dilutions were created by adding 0.5 ml soil solution to the wells (Rusterholz and Mallory 1994). E. coli, a bacterium food source for protozoa was added to each of the wells. Protozoa were separated into the broad categories of Amoebae, Flagellates and Ciliates.
Nematodes were isolated from 20 g of soil samples using Baermann Funnels (Hall 1996). Nematodes were preserved in formalin, counted and identified to functional group based on the morphology of the stoma and stylet (Niles 1994).
Rotifers, Tardigrades, and Enchytraeids were isolated from 5 g of soil using the methods of Peters et al. (1993).
Microarthropods were heat-extracted into 70% ethanol from soils using Tullgren funnels (Moore et al. 2000). The intact sample pairs from each plot were wrapped together in cotton cheesecloth, weighed, placed over a funnel, and heated with a 9-W
incandescent lightbulb for 5 days until dry.
References
Bloem, J. 1995. Fluorescent staining of microbes for total direct counts. In A. D. L. Akkermans, J. D. van Elsas, and F. J. de Bruijn (ed.), Molecular microbial ecology manual. Kluwer Academic Publishers, Dordrecht, The Netherlands. pp. 1-12.
Darbyshire, J. F., R. F. Wheatley, M. P. Greaves, and R. H. E. Inkson. 1974. A rapid micromethod for estimating bacteria and protozoa in soils. Rev. Ecol. Biol. Sol. 11:465-475.
Frey, S.D., E.T. Elliott and K. Paustian. 1999. Bacterial and fungal biomass in conventional and no-tillage agroecosystems along two climatic gradients. Soil Biology and Biochemistry. 31: 573-585.
Hall, G.S. 1996. Methods for the examination of organismal diversity in soils and sediments. CAB International: New York.
Ingham, E.R. 1994. Protozoa. In: R.W. Weaver et al. (ed.), Methods of Soil Analysis. Part 2: Microbiological and Biochemical properties. Soil Science Society of America, Inc., USA. pp. 491-512.
Lodge, D.J. and E.R. Ingham. 1991. A comparison of agar film techniques for estimating fungal biovolumes in litter and soil. Agriculture, Ecosystems and Environment 34: 131-144.
Moore, J. C., B. B. Tripp, R. T. Simpson, and D. C. Coleman. 2000. Springtails in the classroom: Collembola as model
organisms for inquiry-based laboratories. American Biology Teacher 62:512–519.
Newman, E.L. 1966. A method of estimating the total length of root in a sample. J. Appl. Ecol., 3: 139-145.
Niles, R. 1994. Identification of nematode feeding groups. Unpublished manuscript.
Peters, U., Koste, W., and Westheide W. 1993. A quantitative method to extract moss-dwelling rotifers. Hydrobiologia 255-256: 330-341.
Rusterholz, K.J. and L.M. Mallory. 1994. Density, activity, and diversity of bacteria indigenous to a karstic aquifer. Microbial Ecology 28: 79-99.

Sites sampled.

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Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
Site
  • Label: Site
  • Definition: LTER site
  • Type: Nominal
  • Missing values: None specified
Date
  • Label: Date
  • Definition: Date Samples were collected from field
  • Type: Date/time
  • Date format: dd-mmm-yyyy
  • Missing values: None specified
Block
  • Label: Block
  • Definition: Arctic LTER blocks
  • Type: Nominal
  • Missing values: None specified
Fertilization
  • Label: Fertilization
  • Definition: Control vs. N+P since 1996
  • Type: Nominal
  • Missing values: None specified
Grazing
  • Label: Grazing
  • Definition: Control vs. Herbivore exclusion since 1996
  • Type: Nominal
  • Missing values: None specified
Location
  • Label: Location
  • Definition: Tussock vs. inter-Tussock
  • Type: Nominal
  • Missing values: None specified
Bacteria
  • Label: Bacteria
  • Definition: mg bacteria/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Fungi
  • Label: Fungi
  • Definition: mg fungal hyphae/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Flagellates
  • Label: Flagellates
  • Definition: mg flagellates/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Amoebae
  • Label: Amoebae
  • Definition: mg amoebae/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Ciliates
  • Label: Ciliates
  • Definition: mg ciliates/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Bacterivorous Nematodes
  • Label: Bacterivorous Nematodes
  • Definition: mg bacterivorous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Fungivorous Nematodes
  • Label: Fungivorous Nematodes
  • Definition: mg fungivorous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Free Phytophagous Nematodes
  • Label: Free Phytophagous Nematodes
  • Definition: mg free phytophagous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Root-Associated Phytophagous Nematodes
  • Label: Root-Associated Phytophagous Nematodes
  • Definition: mg root associated phytophagous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Total Phytophagous Nematodes
  • Label: Total Phytophagous Nematodes
  • Definition: mg total phytophagous nematode biomass/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Omnivorous Nematodes
  • Label: Omnivorous Nematodes
  • Definition: mg omnivorous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Predatory Nematodes
  • Label: Predatory Nematodes
  • Definition: mg predatory nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Total Rotifers
  • Label: Total Rotifers
  • Definition: mg rotifers/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Tardigrades
  • Label: Tardigrades
  • Definition: mg tardigrades/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Enchytraeids
  • Label: Enchytraeids
  • Definition: mg enchytraeids/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Predatory Mites
  • Label: Predatory Mites
  • Definition: mg predatory mites/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Nematophagous Mites
  • Label: Nematophagous Mites
  • Definition: mg nematophagous mites/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Collembola
  • Label: Collembola
  • Definition: mg collembola/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Cryptostigs
  • Label: Cryptostigs
  • Definition: mg cryptostigs/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Non-Cryptostigs
  • Label: Non-Cryptostigs
  • Definition: mg non-cryptostigs/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing