Dissolved oxygen was measured in sealed chambers on representative river rocks periodically throughout the 2011 and 2012 open water season in the Kuparuk River. These data provide information on gross primary production and respiration from representative river rocks taken from 3 experimental reaches in the Kuparuk.
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Methods used were modified from Bowden et al. (1992) . Photosynthesis and respiration were estimated from linear estimates of change in oxygen within closed chambers during independent light and dark incubations (Bott et al., 1978; Pennak &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; LaVelle, 1979; King, 1982; Duff et al., 1984). Representative river rocks were placed in sealed Plexiglas chambers and slowly filled with river water prior to each incubation. Water was recirculated in the chamber with a stir-bar placed inside of the chamber and powered by a magnetic fans run on batteries on the outside of the chamber. A YSI® LDO probe was inserted into a port at the top of the chamber. Rocks were collected at random locations within riffles at each site. In a typical experiment, three chambers were run simultaneously, with back-to-back light-dark incubations. This was generally done twice to include 6 replicates per site on each date. Rocks were sealed in each chamber then each chamber was submerged into the river as far as possible without water coming over the top of the chamber. Black tops were placed over the chambers during dark incubations to ensure no light would reach the inside of the chamber. Dissolved oxygen and chamber temperature were measured at three minute intervals during the course of the incubation (30-90 minutes). At the end of each experiment, rocks were removed from each chamber and the aluminum foil surface area and weight/area relationships were used to determine surface area of rocks (Steinman et al. 2006). PAR was measured using a Li-Cor Quantum Sensor. Soluble reactive phosphorus was measured using colorimetric method (APHA 1998).
We used 3 experimental reaches in the Kuparuk for this analysis., -0.177k, +0.5k, and 2.0k. In 2011 a phosphorus dripper was added at 0.0k creating fertilized sections downstream from 0.0k to 3.5k. This includes both recovery sections and the fertilized section as indicated by Benstead et al. (2007). University of Alabama Box 870206 Tuscaloosa Alabama 35487 U.S.A
Funding for this project was made available from NSF-OPP#0902126.
APHA (1998) Standard methods for the examination of water and wastewater, American Public Health Association, Washington, DC, USA.
Benstead, J., Green, A., Deegan, L., Peterson, B., Slavik, K., Bowden, W. &amp;amp;amp;amp;amp; Hershey, A. (2007) Recovery of three arctic stream reaches from experimental nutrient enrichment. Freshwater Biology, 52, 1077-1089.
Bowden, W., Peterson, B., Finlay, J. &amp;amp;amp;amp;amp; Tucker, J. (1992) Epilithic chlorophyll a, photosynthesis, and respiration in control and fertilized reaches of a tundra stream. Hydrobiologia, 240, 121-131.
Steinman, A.D., Lamberti, G.A. &amp;amp;amp;amp; Leavitt, P. (2006) Biomass and pigments of benthic algae. Methods in stream ecology, 2.
Bott, T.L., J.R. Brock, C.E. Cushing, S.V. Gregory, D. King &amp;amp;amp; R.C. Petersen, 1978. A comparison of methods for measuring primary productivity and community respiration in streams. Hydrobiologia 60: 3-12.
Duff, J.H,, K.C. Stanley &amp;amp;amp; R.J. Avanzino, 1984. The use of photosynthesis-respiration chambers to measure nitrogen flux in epilithic algal communities. Verh. Internat. Verein. Limnol. 22: 1436-1443.
King, D.K., 1982. Community metabolism and autotrophic-heterotrophic relationships of woodland stream riffle sections. Ph.D. Thesis. Michigan State University, East Lansing. 356 pp.
Pennak, R.W. &amp; J.W. Lavelle, 1979. In situ measurements of net primary production in a Colorado mountain stream. Hydrobiologia 66: 227-235.
Version 1: Package ID assigned and uploaded to web.
Version 2: 29Oct2013 - A. Huryn added as co-owner of data, and funding source added to methods section.
Version 3: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14
Version 4: Changed Distrubution URL since the LTER network DAS system is being discontinued. JimL 9Apr2015
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