Belowground foodweb biomass and soil CN and bulk density from moist acidic tundra nutrient addition plots (since 1989, 2006) sampled July 2011.

Abstract: 

Biomass of belowground community groups (bacteria, fungi, protozoa, nematodes, rotifers, tardigrades) determined for organic and mineral soils in moist acidic tundra. Soil carbon and nitrogen content, bulk density, and depth are included.

Project Keywords: 

Data set ID: 

10459

EML revision ID: 

6

Short name: 

2011_MAT_89_06_NP_FWEB_JCM

DOI: 

10.6073/pasta/a8413b93103e1839681cb1
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Friday, July 1, 2011 to Sunday, July 31, 2011

Publication Date: 

Thursday, December 12, 2013

Methods: 

In July 2011, soil samples were collected from control, and N+P plots from within a set of treatments in Moist Acidic Tundra plots established in 1989. A set of of Control and N+P plots established on an adjacent hillslope in 2006 with both equivalent (high) and half (low) the fertilization rate of the 1989 plots were also sampled. At the time of sample collection we separated the soil into organic horizon, organic/mineral interface, and the upper 5cm of the mineral soil, and measured the depth of each layer. We determined percent moisture for all soils and quantified the biomass of members of the belowground community. Total carbon and nitrogen of all soils were quantified by dry combustion using a Leco TruSpec CN analyzer (Leco Corporation, St. Joseph, Michigan). Direct count slides for microbial biomass estimation were made using the methods of Bloem (1995) as adapted by Frey et al. (1999). 10ml of a soil solution diluted 1:100 (5g initial soil mass) was added to each of 5 6mm diameter wells on a slide. Bacteria slides were stained with DTAF (5-(4, 6 dichlorotriazin-2-yl) aminofluorescein) and Fungi slides were stained with calcifluor M2R fluorescence brightener. Direct counts were made using epifluoresence microscopy. Bacteria were counted, 10 images per well, 2 wells per sample. Fungal hyphal length (Lodge and Ingham 1991) was estimated using the formula of Newman (1966): R=πNA/2H where N=number of hyphae crossing transect line, A=area of well, H=total length of transects. 2 wells, each with 10 images, 3 transects per image were used.
Protozoa densities were estimated from 10 g soil samples using Most Probable Number MPN (Darbyshire et al. 1974; Ingham 1994). 10 g soil was serially diluted 10-1-10-6. Using a 24-well tissue culture plate, four replicate wells of each of the six dilutions were created by adding 0.5 ml soil solution to the wells (Rusterholz and Mallory 1994). E. coli, a bacterium food source for protozoa was added to each of the wells. Protozoa were separated into the broad categories of Amoebae, Flagellates and Ciliates. Nematodes were isolated from 20 g of soil samples using Baermann Funnels (Hall 1996). Nematodes were preserved in formalin, counted and identified to functional group based on the morphology of the stoma and stylet (Niles 1994). Rotifers, Tardigrades, and Enchytraeids were isolated from 5 g of soil using the methods of Peters et al. (1993). Microarthropods were heat-extracted into 70% ethanol from soils using Tullgren funnels (Moore et al. 2000). The intact sample pairs from each plot were wrapped together in cotton cheesecloth, weighed, placed over a funnel, and heated with a 9-W incandescent lightbulb for 5 days until dry.

References
Bloem, J. 1995. Fluorescent staining of microbes for total direct counts. In A. D. L. Akkermans, J. D. van Elsas, and F. J. de Bruijn (ed.), Molecular microbial ecology manual. Kluwer Academic Publishers, Dordrecht, The Netherlands. pp. 1-12.
Darbyshire, J. F., R. F. Wheatley, M. P. Greaves, and R. H. E. Inkson. 1974. A rapid micromethod for estimating bacteria and protozoa in soils. Rev. Ecol. Biol. Sol. 11:465-475.
Frey, S.D., E.T. Elliott and K. Paustian. 1999. Bacterial and fungal biomass in conventional and no-tillage agroecosystems along two climatic gradients. Soil Biology and Biochemistry. 31: 573-585.
Hall, G.S. 1996. Methods for the examination of organismal diversity in soils and sediments. CAB International: New York.
Ingham, E.R. 1994. Protozoa. In: R.W. Weaver et al. (ed.), Methods of Soil Analysis. Part 2: Microbiological and Biochemical properties. Soil Science Society of America, Inc., USA. pp. 491-512.
Lodge, D.J. and E.R. Ingham. 1991. A comparison of agar film techniques for estimating fungal biovolumes in litter and soil. Agriculture, Ecosystems and Environment 34: 131-144.
Moore, J. C., B. B. Tripp, R. T. Simpson, and D. C. Coleman. 2000. Springtails in the classroom: Collembola as model
organisms for inquiry-based laboratories. American Biology Teacher 62:512–519.
Newman, E.L. 1966. A method of estimating the total length of root in a sample. J. Appl. Ecol., 3: 139-145.
Niles, R. 1994. Identification of nematode feeding groups. Unpublished manuscript.
Peters, U., Koste, W., and Westheide W. 1993. A quantitative method to extract moss-dwelling rotifers. Hydrobiologia 255-256: 330-341.
Rusterholz, K.J. and L.M. Mallory. 1994. Density, activity, and diversity of bacteria indigenous to a karstic aquifer. Microbial Ecology 28: 79-99.

Sites sampled.

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Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
Block
  • Label: Block
  • Definition: Arctic LTER blocks
  • Type: Nominal
  • Missing values: None specified
Soil Layer
  • Label: Soil Layer
  • Definition: Soil Layer
  • Type: Nominal
  • Missing values: None specified
Plot Array
  • Label: Plot Array
  • Definition: Distinguished between 1989 and 2006 plots
  • Type: Nominal
  • Missing values: None specified
Fertilization
  • Label: Fertilization
  • Definition: Control vs +NP treatments for each plot array: 1989 +NP = Fertilization treatment since 1989 | 2006 Low+NP = Low fertilization treatment since 2006 | 2006 High+NP = High fertilization treatment since 2006
  • Type: Nominal
  • Missing values: None specified
Bacteria
  • Label: Bacteria
  • Definition: mg bacteria/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Fungi
  • Label: Fungi
  • Definition: mg fungal hyphae/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Flagellates
  • Label: Flagellates
  • Definition: mg flagellates/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Amoebae
  • Label: Amoebae
  • Definition: mg amoebae/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Ciliates
  • Label: Ciliates
  • Definition: mg ciliates/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Bacterivorous Nematodes
  • Label: Bacterivorous Nematodes
  • Definition: mg bacterivorous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Fungivorous Nematodes
  • Label: Fungivorous Nematodes
  • Definition: mg fungivorous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Free Phytophagous Nematodes
  • Label: Free Phytophagous Nematodes
  • Definition: mg free phytophagous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Root-Associated Phytophagous Nematodes
  • Label: Root-Associated Phytophagous Nematodes
  • Definition: mg root associated phytophagous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Total Phytophagous Nematodes
  • Label: Total Phytophagous Nematodes
  • Definition: mg total phytophagous nematode biomass/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Omnivorous Nematodes
  • Label: Omnivorous Nematodes
  • Definition: mg omnivorous nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Predatory Nematodes
  • Label: Predatory Nematodes
  • Definition: mg predatory nematodes/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Total Rotifers
  • Label: Total Rotifers
  • Definition: mg rotifers/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Tardigrades
  • Label: Tardigrades
  • Definition: mg tardigrades/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Enchytraeids
  • Label: Enchytraeids
  • Definition: mg enchytraeids/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Predatory Mites
  • Label: Predatory Mites
  • Definition: mg predatory mites/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Nematophagous Mites
  • Label: Nematophagous Mites
  • Definition: mg nematophagous mites/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Collembola
  • Label: Collembola
  • Definition: mg collembola/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Cryptostigs
  • Label: Cryptostigs
  • Definition: mg cryptostigs/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Non-Cryptostigs
  • Label: Non-Cryptostigs
  • Definition: mg non-cryptostigs/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Soil Carbon
  • Label: Soil Carbon
  • Definition: mg Carbon/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Soil Nitrogen
  • Label: Soil Nitrogen
  • Definition: mg Nitrogen/g dry soil
  • Type: Physical quantity
  • Unit: milligramPerGram
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Bulk Density
  • Label: Bulk Density
  • Definition: Soil Bulk Density
  • Type: Physical quantity
  • Unit: gramPerCentimeterCubed
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -9999 = missing
Layer Depth
  • Label: Layer Depth
  • Definition: Depth of soil Layer
  • Type: Physical quantity
  • Unit: centimeter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • -999 = missing
    • 20+ = depth to permafrost exceeded 20cm