Number of cyanobacteria in Toolik Lake at 1 meter depth during June, July and August 1996 , Arctic LTER, summer 1996.

Abstract: 

Number of cyanobacteria in Toolik Lake at 1 meter depth during June, July and August 1996. Samples were transported to the Dept. of Fisheries and Oceans in West Vancouver, British Columbia, Canada for analysis.

Project Keywords: 

Data set ID: 

969

EML revision ID: 

8

Short name: 

96hocyan

DOI: 

10.6073/pasta/3ee39ca86220c42d24edb21238d62e2f
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Wednesday, May 1, 1996 to Sunday, September 1, 1996

Publication Date: 

Saturday, December 12, 1998

Methods: 

Lake water was collected from the Toolik Lake main station at 1 m depth and preserved with 2%
glutaraldehyde. Samples were transported to the Dept. of Fisheries and Oceans in West Vancouver, British
Columbia, Canada for analysis. Twenty ml aliquots were filtered onto 0.2 black polycarbonate membrane
using a 25 mm filterholder, with vacuum pressure kept to less than 5 kPa. Filters were transferred to a
microscope slide and mounted with nonfluorescent immersion oil. Cells were counted at 1000x
magnification using a green-yellow (520-560 nm) excitation filter with an epifluorescence microscope.
Cell volumes were estimated by measuring the dimensions of subsets of cells with an eyepiece micrometer.
Calculations for cell volumes were done using formulae for geometric shapes approximating the shapes of
the cells. Synechococcus and Microcolonial Synechococcus assumed spheres with average cell
diamter 0.9 mm; Oscillatoria assumed cylinder with 0.8 mm width x average length.

Reference Citations: MacIsaac, E.A. and J.G. Stockner. 1993. Enumeration of phototrophic picoplankton by autofluorescence
microscopy. In Kemp, P., B.F. Sherr, E.B. Sherr and J.J. Cole (eds.) Handbook of Methods in Aquatic
Microbial Ecology, lewis Publishers, Boca Raton, pp. 187-197.

Sites sampled.

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File 96hocyan.csv

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Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
Site Code
  • Label: Site Code
  • Definition: Site Code, LTER code
  • Type: Nominal
  • Missing values: None specified
Site
  • Label: Site
  • Definition: Site name
  • Type: Nominal
  • Missing values: None specified
Date
  • Label: Date
  • Definition: Date
  • Type: Date/time
  • Date format: dd-mmm-yyyy
  • Missing values:
    • . = Missing or Not Measured
Depth (m)
  • Label: Depth (m)
  • Definition: meters
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Cell type
  • Label: Cell type
  • Definition: Genus
  • Type: Nominal
  • Missing values: None specified
Biovolume (um^3)
  • Label: Biovolume (um^3)
  • Definition: Average cell biovolume in um3
  • Type: Physical quantity
  • Unit: micrometerCubed
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Count (3/ml)
  • Label: Count (3/ml)
  • Definition: Number of cells per ml
  • Type: Physical quantity
  • Unit: numberPerMilliliter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Biomass (um^3/ml)
  • Label: Biomass (um^3/ml)
  • Definition: Micrometers cubed per milliliter
  • Type: Physical quantity
  • Unit: micrometerCubedPerMilliliter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured