uid=ARC,o=EDI,dc=edirepository,dc=org all public read RF_2006_dissolved_nuts Phosphate and ammonium from experiemental plots near Toolik Lake, AK from 2006 George Kling University of Michigan
University of Michigan,Department of Ecology and Evolutionary Biology, 830 North University Ann Arbor MI 48109-1048 US
734 647 0894 gwk@umich.edu https://lsa.umich.edu/eeb/people/faculty/gwk.html https://orcid.org/0000-0002-6349-8227
Knute Nadelhoffer
830 N. University, Department of Ecology and Evolutionary Biology Ann Arbor MI 48019-1048 US
Martin Sommerkorn
US
Edward Rastetter
7 M B L St. Woods Hole MA 02543 US
erastetter@mbl.edu https://www.mbl.edu/ecosystems/rastetter/ https://orcid.org/0000-0002-8620-5431 associated researcher
Loretta Johnson
US
associated researcher
Suzanne Randazzo
US
associated researcher
Amy Anstead
US
associated researcher
Caren Scott
US
associated researcher
Jen Kostrzewski
MI US
associated researcher
Tyler Suelter
US
associated researcher
2008 English
Soluble reactive phosphorus (SRP) and ammonium in waters from tussock tundra and wet sedge plots near Toolik Lake, AK during the summer of 2006.
soluble reactive phosphorus phosphate nutrients ammonium LTER Controlled Vocabulary arctic soils moist acidic tundra wet sedge tundra Arctic LTER Vocabulary
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https://arc-lter.ecosystems.mbl.edu/rf2006dissolvednuts LTER Moist Acidic Tussock Tundra: Arctic LTER Experimental Plots: Moist Acidic Tussock Tundra (MAT) Northeast corner block 1 near Toolik Field Station, North Slope, Alaska. -149.610000610352 -149.610000610352 68.624397277832 68.624397277832 750 750 meter LTER Wet Sedge Tundra Block 2: Arctic LTER Experimental Plots: Wet Sedge Tundra Block 2 Northeast corner near Toolik Field Station, North Slope, Alaska. -149.576995849609 -149.576995849609 68.647598266602 68.647598266602 719 719 meter 2006-08-02 2006-08-03 Entered metadata into new metadata form. Version 2: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 27Jan14 Arctic_LTER Information Manager Arctic Long Term Ecological Research
7 M B L St Woods Hole MA 02536 US
(508) 548-3705 arc_im@mbl.edu
Arctic Long Term Ecological Research
Plot location was chosen based on dominate vegetation type (T or W). The tussock tundra plots are located near the Moist Acidic Tundra plots characterized by Shaver and Chapin (1991). The wet sedge plots are located near the outlet of Toolik Lake; all plots have been used by the Arctic LTER project at Toolik Lake. In wet sedge tundra, three 1 m2-sized plots were positioned randomly in each of two treatments of block 2 of the LTER wet sedge scheme (outlet site), control and N+P-fertilized , respectively, in 2000. Paired control and fertilized plots were labelled with 14C and 15N at one of three different dates of the summer season 2001. In moist acidic tussock tundra six 1 m2 sized plots were positioned in three of the "X" (=extra") blocks of the LTER scheme, in 2001, inside an area extending 5 m from the boardwalks and, positioning plots so that similar area shares between tussocks and intertussock areas was achieved. In each of the three X blocks two 1m2-sized plots were established. The whole 5 m area of the X blocks extending from the board walks was divided into two halfs, each containing one of the 1m2 sized plots. One half was set aside as the control and the other half was designated to be fertilized. Fertilization with a Hoagland solution (see protocol) started in late summer 2001, and was repeated annually after that , early in the summer season. Paired control and fertilized plots were labelled with 14C and 15N of the summer season 2002; all three pairs were labelled as closely together in time as logistically possible. Using soil needles and syringes, we pulled soil water for dissolved nutrient analyses. The Water was filtered using a Whatman GF/F filter (0.7μm pore size, nominal) into polypropelyne bottles (SRP and NH4) or glass scint vials (DOC). Samples were stored at 4 degreeC in the dark until analysis. SRP and NH4 were analyzed usually within 24hrs of sampling. DOC samples were acidified to pH ~3 after filtration and sent to the University of Michigan for analysis. 1. SRP: Soluble reactive phosphorus was analyzed using a molybdate colorometric assay (Wetzel and Likens, 1979). Each sample, standard, and blank was duplicated during analysis. We pipetted 10 mLs of each into two P tubes. After the 1 mL SRP reagent was added the samples were incubated in the tlight for 30-90 minutes. The samples were analyzed on the spectrophotometer in the dry lab. All waste was dumped into radactive waste container. 2. NH4: Ammonium ion concentrations were analyzed using a fluorometric assay modified to correct the calculation errors in the original publication. Details of methods, reagents, etc are given in Holmes et al. 1999. The method relies on the the reagent Orthophthadialdehyde (OPA) to bind witht he ammonium causing fluorescence. Each sample under went four treatments: 1. Background Fluorescence (BF), sample + color blank, 2. Sample + Working Reagent, 3. Sample + Working Reagent + Low Spike Addition, and 4. Sample + Working Reagent + Low Spike Addition. The samples were incubated in the dark for at least 8 hours. All treatments were measured on the flourometer in the wet lab. Background fluorescence was subtracted from treatments 2,3,and 4. The concentration of ammonium was calculated using a standard addition curve to account for Matrix Effects (APHA, 1995). All waste was dumped into radactive waste container. 3. DOC: Samples were run on a Shimadzu TOC 5000 Carbon Analyzer using a platinum catalyzed high-temperature combustion to CO2 and infrared detection (Shimadzu TOC-5000; Shimadzu, Columbia, MD). American Public Health Association. 1995. Standard Methods for the Examination of Water and Wastewater, 19th Edition. American Public Health Association. USA. Holmes R.M., Aminot A., Kerouel R., Hooker, B.A., Peterson, B.J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Canadian Journal of Fisheries and Aquatic Sciences 56 (10): 1801-1808. Shaver, G.R., and F.S. Chapin. 1991. Production - biomass relationships and elemental cycling in contrasting arctic vegetation types. Ecological Monographs 61(1): 1-31. Wetzel, R.G. and G.E. Likens. 1979. Limnological Analyses. 2nd ed. Springer-Verlag, New York. pp 89-92.
RF_2006_dissolved_nuts_csv Phosphate and ammonium from experiemental plots near Toolik Lake, AK from 2006 RF_2006_dissolved_nuts.csv 675 ac32d2836f3ae58e0f4743d5008a85ca 77269d5d7fd8defa210c296b357874aa324ef6c7 1 0 \r\n column , " https://arc-lter.ecosystems.mbl.edu/sites/default/files/data/root_dynamics/RF_2006_dissolved_nuts.csv Vegetation Vegetation Vegetation Type: t = tussock tundra | w = wet sedge string Vegetation Type: t = tussock tundra | w = wet sedge Treatment Treatment Treatment Type: c = control | f = fertilized string Treatment Type: c = control | f = fertilized Plot# Plot# Plot Number: 1 = plot 1 | 2 = plot 2 | 3 = plot 3 string Plot Number: 1 = plot 1 | 2 = plot 2 | 3 = plot 3 Section Section Location sampled within the plot: for tussock, t = tussock | for tussock, n = inner tussock | for wet sedge, n = north | for wet sedge, s = south string Location sampled within the plot: for tussock, t = tussock | for tussock, n = inner tussock | for wet sedge, n = north | for wet sedge, s = south Round Round Replicate Identification: a = 1st replicate | b = 2nd replicate | c = 3rd replicate string Replicate Identification: a = 1st replicate | b = 2nd replicate | c = 3rd replicate Date_sampled Date_sampled Date sampled date dd-mmm-yy . Missing or Not Measured Time Time Time sampled date hh:mm . Missing or Not Measured Depth Depth Depth of Sample meter real . Missing or Not Measured Type Type Type of sample: d-nut = Dissolved nutrient sample string Type of sample: d-nut = Dissolved nutrient sample PO4_uM PO4_uM SRP Concentration of Sample micromolePerLiter real . Missing or Not Measured NH4_uM NH4_uM Ammonium Concentration of Sample micromolePerLiter real . Missing or Not Measured 12 RF_2006_dissolved_nuts_excel Excel file with metadata and data sheets. Phosphate and ammonium from experiemental plots near Toolik Lake, AK from 2006 RF_2006_dissolved_nuts.xls 413184 4e36975b5406926f06452a1a60f3ad82 8c83c7b711c916296a48843f12a19d547f972d05 application/vnd.ms-excel https://arc-lter.ecosystems.mbl.edu/sites/default/files/data/root_dynamics/RF_2006_dissolved_nuts.xls document
micromoles per liter of solution