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RF_2006_dissolved_nuts
Phosphate and ammonium from experiemental plots near Toolik Lake, AK from 2006
George
Kling
University of Michigan
University of Michigan,Department of Ecology and Evolutionary Biology, 830 North University
Ann Arbor
MI
48109-1048
US
734 647 0894
gwk@umich.edu
https://lsa.umich.edu/eeb/people/faculty/gwk.html
https://orcid.org/0000-0002-6349-8227
Knute
Nadelhoffer
830 N. University, Department of Ecology and Evolutionary Biology
Ann Arbor
MI
48019-1048
US
Martin
Sommerkorn
US
Edward
Rastetter
7 M B L St.
Woods Hole
MA
02543
US
erastetter@mbl.edu
https://www.mbl.edu/ecosystems/rastetter/
https://orcid.org/0000-0002-8620-5431
associated researcher
Loretta
Johnson
US
associated researcher
Suzanne
Randazzo
US
associated researcher
Amy
Anstead
US
associated researcher
Caren
Scott
US
associated researcher
Jen
Kostrzewski
MI
US
associated researcher
Tyler
Suelter
US
associated researcher
2008
English
Soluble reactive phosphorus (SRP) and ammonium in waters from tussock tundra and wet sedge plots near Toolik Lake, AK during the summer of 2006.
soluble reactive phosphorus
phosphate
nutrients
ammonium
LTER Controlled Vocabulary
arctic soils
moist acidic tundra
wet sedge tundra
Arctic LTER Vocabulary
Data Policies
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.
Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User should properly cite the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set.
Acknowledgement. The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed significantly to its content. Acknowledgements should identify the supporting party, the party that received the support, and any identifying information such as grant numbers.
For example: Data sets were provided by the Arctic LTER. This material is based upon work supported by the National Science Foundation under Award numbers #1637459,1026843, 9810222, 9211775, 8702328.
https://arc-lter.ecosystems.mbl.edu/rf2006dissolvednuts
LTER Moist Acidic Tussock Tundra: Arctic LTER Experimental Plots: Moist Acidic Tussock Tundra (MAT) Northeast corner block 1 near Toolik Field Station, North Slope, Alaska.
-149.610000610352
-149.610000610352
68.624397277832
68.624397277832
750
750
meter
LTER Wet Sedge Tundra Block 2: Arctic LTER Experimental Plots: Wet Sedge Tundra Block 2 Northeast corner near Toolik Field Station, North Slope, Alaska.
-149.576995849609
-149.576995849609
68.647598266602
68.647598266602
719
719
meter
2006-08-02
2006-08-03
Entered metadata into new metadata form.
Version 2: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 27Jan14
Arctic_LTER
Information Manager
Arctic Long Term Ecological Research
7 M B L St
Woods Hole
MA
02536
US
(508) 548-3705
arc_im@mbl.edu
Arctic Long Term Ecological Research
Plot location was chosen based on dominate vegetation type (T or W). The tussock tundra plots are located near the Moist Acidic Tundra plots characterized by Shaver and Chapin (1991). The wet sedge plots are located near the outlet of Toolik Lake; all plots have been used by the Arctic LTER project at Toolik Lake.
In wet sedge tundra, three 1 m2-sized plots were positioned randomly in each of two treatments of block 2 of the LTER wet sedge scheme (outlet site), control and N+P-fertilized , respectively, in 2000. Paired control and fertilized plots were labelled with 14C and 15N at one of three different dates of the summer season 2001.
In moist acidic tussock tundra six 1 m2 sized plots were positioned in three of the "X" (=extra") blocks of the LTER scheme, in 2001, inside an area extending 5 m from the boardwalks and, positioning plots so that similar area shares between tussocks and intertussock areas was achieved. In each of the three X blocks two 1m2-sized plots were established. The whole 5 m area of the X blocks extending from the board walks was divided into two halfs, each containing one of the 1m2 sized plots. One half was set aside as the control and the other half was designated to be fertilized. Fertilization with a Hoagland solution (see protocol) started in late summer 2001, and was repeated annually after that , early in the summer season. Paired control and fertilized plots were labelled with 14C and 15N of the summer season 2002; all three pairs were labelled as closely together in time as logistically possible.
Using soil needles and syringes, we pulled soil water for dissolved nutrient analyses. The Water was filtered using a Whatman GF/F filter (0.7μm pore size, nominal) into polypropelyne bottles (SRP and NH4) or glass scint vials (DOC). Samples were stored at 4 degreeC in the dark until analysis. SRP and NH4 were analyzed usually within 24hrs of sampling. DOC samples were acidified to pH ~3 after filtration and sent to the University of Michigan for analysis.
1. SRP:
Soluble reactive phosphorus was analyzed using a molybdate colorometric assay (Wetzel and Likens, 1979). Each sample, standard, and blank was duplicated during analysis. We pipetted 10 mLs of each into two P tubes. After the 1 mL SRP reagent was added the samples were incubated in the tlight for 30-90 minutes. The samples were analyzed on the spectrophotometer in the dry lab. All waste was dumped into radactive waste container.
2. NH4:
Ammonium ion concentrations were analyzed using a fluorometric assay modified to correct the calculation errors in the original publication. Details of methods, reagents, etc are given in Holmes et al. 1999. The method relies on the the reagent Orthophthadialdehyde (OPA) to bind witht he ammonium causing fluorescence. Each sample under went four treatments: 1. Background Fluorescence (BF), sample + color blank, 2. Sample + Working Reagent, 3. Sample + Working Reagent + Low Spike Addition, and 4. Sample + Working Reagent + Low Spike Addition. The samples were incubated in the dark for at least 8 hours. All treatments were measured on the flourometer in the wet lab. Background fluorescence was subtracted from treatments 2,3,and 4. The concentration of ammonium was calculated using a standard addition curve to account for Matrix Effects (APHA, 1995). All waste was dumped into radactive waste container.
3. DOC:
Samples were run on a Shimadzu TOC 5000 Carbon Analyzer using a platinum catalyzed high-temperature combustion to CO2 and infrared detection (Shimadzu TOC-5000; Shimadzu, Columbia, MD).
American Public Health Association. 1995. Standard Methods for the Examination of Water and Wastewater, 19th Edition. American Public Health Association. USA.
Holmes R.M., Aminot A., Kerouel R., Hooker, B.A., Peterson, B.J. 1999. A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Canadian Journal of Fisheries and Aquatic Sciences 56 (10): 1801-1808.
Shaver, G.R., and F.S. Chapin. 1991. Production - biomass relationships and elemental cycling in contrasting arctic vegetation types. Ecological Monographs 61(1): 1-31.
Wetzel, R.G. and G.E. Likens. 1979. Limnological Analyses. 2nd ed. Springer-Verlag, New York. pp 89-92.
RF_2006_dissolved_nuts_csv
Phosphate and ammonium from experiemental plots near Toolik Lake, AK from 2006
RF_2006_dissolved_nuts.csv
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ac32d2836f3ae58e0f4743d5008a85ca
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https://arc-lter.ecosystems.mbl.edu/sites/default/files/data/root_dynamics/RF_2006_dissolved_nuts.csv
Vegetation
Vegetation
Vegetation Type: t = tussock tundra | w = wet sedge
string
Vegetation Type: t = tussock tundra | w = wet sedge
Treatment
Treatment
Treatment Type: c = control | f = fertilized
string
Treatment Type: c = control | f = fertilized
Plot#
Plot#
Plot Number: 1 = plot 1 | 2 = plot 2 | 3 = plot 3
string
Plot Number: 1 = plot 1 | 2 = plot 2 | 3 = plot 3
Section
Section
Location sampled within the plot: for tussock, t = tussock | for tussock, n = inner tussock | for wet sedge, n = north | for wet sedge, s = south
string
Location sampled within the plot: for tussock, t = tussock | for tussock, n = inner tussock | for wet sedge, n = north | for wet sedge, s = south
Round
Round
Replicate Identification: a = 1st replicate | b = 2nd replicate | c = 3rd replicate
string
Replicate Identification: a = 1st replicate | b = 2nd replicate | c = 3rd replicate
Date_sampled
Date_sampled
Date sampled
date
dd-mmm-yy
.
Missing or Not Measured
Time
Time
Time sampled
date
hh:mm
.
Missing or Not Measured
Depth
Depth
Depth of Sample
meter
real
.
Missing or Not Measured
Type
Type
Type of sample: d-nut = Dissolved nutrient sample
string
Type of sample: d-nut = Dissolved nutrient sample
PO4_uM
PO4_uM
SRP Concentration of Sample
micromolePerLiter
real
.
Missing or Not Measured
NH4_uM
NH4_uM
Ammonium Concentration of Sample
micromolePerLiter
real
.
Missing or Not Measured
12
RF_2006_dissolved_nuts_excel
Excel file with metadata and data sheets. Phosphate and ammonium from experiemental plots near Toolik Lake, AK from 2006
RF_2006_dissolved_nuts.xls
413184
4e36975b5406926f06452a1a60f3ad82
8c83c7b711c916296a48843f12a19d547f972d05
application/vnd.ms-excel
https://arc-lter.ecosystems.mbl.edu/sites/default/files/data/root_dynamics/RF_2006_dissolved_nuts.xls
document
micromoles per liter of solution