uid=ARC,o=EDI,dc=edirepository,dc=org all public read 1996_bacprods_Kling Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 1996, Arctic LTER, Toolik Research Station, Alaska. George Kling University of Michigan
University of Michigan,Department of Ecology and Evolutionary Biology, 830 North University Ann Arbor MI 48109-1048 US
734 647 0894 gwk@umich.edu https://lsa.umich.edu/eeb/people/faculty/gwk.html https://orcid.org/0000-0002-6349-8227
Arctic LTER MBL
7 M B L St. Woods Hole MA 02543 US
arc_im@mbl.edu
1998 English
Yearly file containing information on bacterial productivity. Samples were collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number (sortchem), site, date, time and depth.
populations Core Areas bacteria bacterial production carbon carbon assimilation communities lakes populations streams LTER Controlled Vocabulary 14C 14C-leucine arctic lakes arctic streams inlet stream transplant Arctic LTER Vocabulary
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https://arc-lter.ecosystems.mbl.edu/1996bacprodskling Toolik Main: Toolik Lake Main Station -149.612640380859 -149.612640380859 68.629959106445 68.629959106445 719 719 meter Toolik Inlet: Arctic LTER Site number 13 -149.595993041992 -149.595993041992 68.625602722168 68.625602722168 719 719 meter TW Weir: Tussock Experimental Watershed Weir -149.617004394531 -149.617004394531 68.623497009277 68.623497009277 761 761 meter 1996-05-13 1996-08-21 Entered metadata into new metadata form. Version 3: Data URL was changed to point to the LTERNET Data Access server Version 10: Updated metadata form to newer version (without site sheet). Fixed discrepency with header variable names. CH 1Feb2013 Updated metadata form to newer version (with site sheet) CH March 2013. Time format updated, new urls and eml files for web created JD-Nov2013 Version 11: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14 Version 12: Changed Distrubution URL since the LTER network DAS system is being discontinued. JimL 9Apr2015 Updated data file from Kling - Using only Toolik Main, Toolik Inlet and TW Weir. Mar 2016 JimL Arctic_LTER Information Manager Arctic Long Term Ecological Research
7 M B L St Woods Hole MA 02536 US
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Arctic LTER MBL
7 M B L St. Woods Hole MA 02543 US
arc_im@mbl.edu
Arctic Long Term Ecological Research
For the bacterial production protocol, see the Land-Water protocol accessible from the Landscape Interaactionr mainpage on the Arctic LTER web page or at http://www-personal.umich.edu/~gwk and clicking on the protocol link at the bottom of the page. Bacterial production is determined by the incorporation of 13C labeled Leucine (Simon and Azam 1989). Water samples are vacuum filtered through 0.22 um Millapore GS cellulose nitrate filters. Samples are extracted with 5mL of 5% TCA for 5 minutes, rinsed with additional TCA, and air dried in mini-scintillation vials. Following the addition of 1 mL cellusolve, filters are left to dissolve overnight. Scinti-safe scintillation cocktail (4 mL) is then added to each vial and samples are counted at Toolik Field Station on a liquid scintillation counter (Packard Tri-Carb 2100TR) . Calculations of bacterial productivity assume that the Internal Dilution (ID) of leucine is 1, but studies show it may range from 1-2 (Kirchman 1993). Some published data from these LTER files have used an ID value different than 1; in those studies, the ID value used is stated in the article. 1. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series 51:201-213. 2. Kirchmann, D.L. 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. Pages 509-512 in P.F. Kemp, B.F. Sherr, E.B. Sherr, and J.J. Cole, editors. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Florida, USA. http://arc.lternet.edu/landwater/lw_protocols
1996_bacprods_Kling_csv Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 1996, Arctic LTER, Toolik Research Station, Alaska. 1996_bacprods_Kling.csv 1996 0b5864d3cbe6e0eab722f8db2e27b2f1 1948f9f84c383068a6391984f7febd02cfc8462d 1 0 \r\n column , " https://arc-lter.ecosystems.mbl.edu/sites/default/files/data/landwater/1996_bacprods_Kling.csv SortChem SortChem A unique number assigned to each sample collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600. string A unique number assigned to each sample collected at a specific site at a specific date and time. The year that the sample was collected is followed by a dash and then a sequential number. For example, if 600 samples were collected in 2002, then the first sample collected is 2002-0001 and the last sample is 2002-0600. Site Site Site Name. If a soil site, the site is described in the name by general location then specific feature (e.g., watertrack) then specific sampling point (1,2,3,...). Lake and stream sites use common names (e.g., Toolik) or LTER number codes. Exceptions in naming procedure are provided in the Code Information and in the AK-LTER_Site_Info.xls file. string Site Name. If a soil site, the site is described in the name by general location then specific feature (e.g., watertrack) then specific sampling point (1,2,3,...). Lake and stream sites use common names (e.g., Toolik) or LTER number codes. Exceptions in naming procedure are provided in the Code Information and in the AK-LTER_Site_Info.xls file. Date Date Sampling Date date dd-mmm-yy . Missing or Not Measured Time_hr_dst Time_hr_dst Sampling Time in Alaska Daylight Savings Time (1 hour ahead of Alaska Standard Time: if 13:00 DST than 12:00 AST) date hh:mm . Missing or Not Measured Depth_m Depth_m Depth from surface of lake, stream, or soil meter real . Missing or Not Measured Bac_prod_ug C_L_day Bac_prod_ug C_L_day Bacterial Productivity, ug Carbon per L per day microgramPerLiterPerDay real . Missing or Not Measured 47 1996_bacprods_Kling_excel Excel file with sheets for metadata and data. _x000D_ Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 1996, Arctic LTER, Toolik Research Station, Alaska. 1996_bacprods_Kling.xlsx 102825 7529538441e938c128231a26b0d6d691 3089f81dc7b1c0e4478fddb5c69ff437443b03fa application/vnd.openxmlformats-officedocument.spreadsheetml.sheet https://arc-lter.ecosystems.mbl.edu/sites/default/files/data/landwater/1996_bacprods_Kling.xlsx document
micrograms per (liter per day)