Microbial respiration of carbon dioxide, and methane in waters from wet sedge plots near Toolik Lake, AK during the summer of 2001.
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Plot location was chosen based on dominate vegetation type (T or W). The tussock tundra plots are located near the Moist Acidic Tundra plots characterized by Shaver and Chapin (1991). The wet sedge plots are located near the outlet of Toolik Lake; all plots have been used by the Arctic LTER project at Toolik Lake.
In wet sedge tundra, three 1 m2-sized plots were positioned randomly in each of two treatments of block 2 of the LTER wet sedge scheme (outlet site), control and N+P-fertilized , respectively, in 2000. Paired control and fertilized plots were labelled with 14C and 15N at one of three different dates of the summer season 2001.
In moist acidic tussock tundra six 1 m2 sized plots were positioned in three of the "X" (=extra") blocks of the LTER scheme, in 2001, inside an area extending 5 m from the boardwalks and, positioning plots so that similar area shares between tussocks and intertussock areas was achieved. In each of the three X blocks two 1m2-sized plots were established. The whole 5 m area of the X blocks extending from the board walks was divided into two halfs, each containing one of the 1m2 sized plots. One half was set aside as the control and the other half was designated to be fertilized. Fertilization with a Hoagland solution (see protocol) started in late summer 2001, and was repeated annually after that , early in the summer season. Paired control and fertilized plots were labelled with 14C and 15N of the summer season 2002; all three pairs were labelled as closely together in time as logistically possible.
From these plots we collected soil cores, which we removed the root material. The a portion of the cores were placed in sealed mason jars. We fumigated the blank to remove the influce of microbial communities. At set intervals (1 day, 7 days, 3 weeks) we collected the air in the headspace of the mason jar and analyzed on a Shimadzu model 14A gas chromatograph with a flame ionization detector (FID) for CO2 and CH4 content.
Shaver, G.R., and F.S. Chapin. 1991. Production - biomass relationships and elemental cycling in contrasting arctic vegetation types. Ecological Monographs 61(1): 1-31.
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