Microbial Respirations from experiemental plots near Toolik Lake, AK for 2001

Abstract: 

Microbial respiration of carbon dioxide, and methane in waters from wet sedge plots near Toolik Lake, AK during the summer of 2001.

Project Keywords: 

Data set ID: 

10359

EML revision ID: 

3

Short name: 

RF_2001_Microbial

DOI: 

10.6073/pasta/378a6db184e7
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Friday, June 29, 2001 to Saturday, August 18, 2001

Publication Date: 

Friday, December 12, 2003

Methods: 

Plot location was chosen based on dominate vegetation type (T or W). The tussock tundra plots are located near the Moist Acidic Tundra plots characterized by Shaver and Chapin (1991). The wet sedge plots are located near the outlet of Toolik Lake; all plots have been used by the Arctic LTER project at Toolik Lake.

In wet sedge tundra, three 1 m2-sized plots were positioned randomly in each of two treatments of block 2 of the LTER wet sedge scheme (outlet site), control and N+P-fertilized , respectively, in 2000. Paired control and fertilized plots were labelled with 14C and 15N at one of three different dates of the summer season 2001.

In moist acidic tussock tundra six 1 m2 sized plots were positioned in three of the "X" (=extra") blocks of the LTER scheme, in 2001, inside an area extending 5 m from the boardwalks and, positioning plots so that similar area shares between tussocks and intertussock areas was achieved. In each of the three X blocks two 1m2-sized plots were established. The whole 5 m area of the X blocks extending from the board walks was divided into two halfs, each containing one of the 1m2 sized plots. One half was set aside as the control and the other half was designated to be fertilized. Fertilization with a Hoagland solution (see protocol) started in late summer 2001, and was repeated annually after that , early in the summer season. Paired control and fertilized plots were labelled with 14C and 15N of the summer season 2002; all three pairs were labelled as closely together in time as logistically possible.

From these plots we collected soil cores, which we removed the root material. The a portion of the cores were placed in sealed mason jars. We fumigated the blank to remove the influce of microbial communities. At set intervals (1 day, 7 days, 3 weeks) we collected the air in the headspace of the mason jar and analyzed on a Shimadzu model 14A gas chromatograph with a flame ionization detector (FID) for CO2 and CH4 content.

Shaver, G.R., and F.S. Chapin. 1991. Production - biomass relationships and elemental cycling in contrasting arctic vegetation types. Ecological Monographs 61(1): 1-31.

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

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Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
Vegetation
  • Label: Vegetation
  • Definition: Vegetation Type where t= tussock, w = wet sedge
  • Type: Nominal
  • Missing values: None specified
Treatment
  • Label: Treatment
  • Definition: Treatment Type where c = control, f = fertilized (N+P)
  • Type: Nominal
  • Missing values: None specified
Plot#
  • Label: Plot#
  • Definition: Plot Number (1, 2, or 3)
  • Type: Nominal
  • Missing values: None specified
Incubation Period
  • Label: Incubation Period
  • Definition: Incubation Time Period in days (1d = one day etc.)
  • Type: Nominal
  • Missing values: None specified
Replicate
  • Label: Replicate
  • Definition: Replicate Identification where Blank = fumigated
  • Type: Nominal
  • Missing values: None specified
Date
  • Label: Date
  • Definition: Date sampled
  • Type: Date/time
  • Date format: dd-mmm-yyyy
  • Missing values:
    • . = Missing or Not Measured
Time
  • Label: Time
  • Definition: Time sampled
  • Type: Date/time
  • Date format: hh:mm
  • Missing values:
    • . = Missing or Not Measured
Category
  • Label: Category
  • Definition: Type of sample where mr = microbial respiration
  • Type: Nominal
  • Missing values: None specified
Elev_m
  • Label: Elev_m
  • Definition: Elevation of the sample site
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Depth_m
  • Label: Depth_m
  • Definition: Depth of the sample site in meters
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
PCO2_uatm
  • Label: PCO2_uatm
  • Definition: Partial Pressure of Carbon Dioxide
  • Type: Physical quantity
  • Unit: microatmosphere
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
PCH4_uatm
  • Label: PCH4_uatm
  • Definition: Partial Pressure of Methane
  • Type: Physical quantity
  • Unit: microatmosphere
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
CO2_umol/L
  • Label: CO2_umol/L
  • Definition: Concentration of Carbon Dioxide
  • Type: Physical quantity
  • Unit: micromolePerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
CH4_umol/L
  • Label: CH4_umol/L
  • Definition: Concentration of Methane
  • Type: Physical quantity
  • Unit: micromolePerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured