Pre-labeled dissolved inorganic carbon, carbon dioxide, and methane from experiemental plots near Toolik Lake, AK from 2006

Abstract: 

Dissolved inorganic carbon, carbon dioxide, and methane in waters from pre-labeled wet sedge plots near Toolik Lake, AK during the summer of 2006.

Project Keywords: 

Data set ID: 

10389

EML revision ID: 

3

Short name: 

RF_2006_dissolved_gas

DOI: 

10.6073/pasta/5
People

Keywords: 

Arctic LTER keywords: 

Dates

Date Range: 

Wednesday, August 2, 2006 to Thursday, August 3, 2006

Publication Date: 

Friday, December 12, 2008

Methods: 

Plot location was chosen based on dominate vegetation type (T or W). The tussock tundra plots are located near the Moist Acidic Tundra plots characterized by Shaver and Chapin (1991). The wet sedge plots are located near the outlet of Toolik Lake; all plots have been used by the Arctic LTER project at Toolik Lake.

In wet sedge tundra, three 1 m2-sized plots were positioned randomly in each of two treatments of block 2 of the LTER wet sedge scheme (outlet site), control and N+P-fertilized , respectively, in 2000. Paired control and fertilized plots were labelled with 14C and 15N at one of three different dates of the summer season 2001.

In moist acidic tussock tundra six 1 m2 sized plots were positioned in three of the "X" (=extra") blocks of the LTER scheme, in 2001, inside an area extending 5 m from the boardwalks and, positioning plots so that similar area shares between tussocks and intertussock areas was achieved. In each of the three X blocks two 1m2-sized plots were established. The whole 5 m area of the X blocks extending from the board walks was divided into two halfs, each containing one of the 1m2 sized plots. One half was set aside as the control and the other half was designated to be fertilized. Fertilization with a Hoagland solution (see protocol) started in late summer 2001, and was repeated annually after that , early in the summer season. Paired control and fertilized plots were labelled with 14C and 15N of the summer season 2002; all three pairs were labelled as closely together in time as logistically possible.

1. Dissolved Gas Sampling Description:

Using soil sampling needles and a 60 mL syringe, we pulled 40 mL of bubble-free water out of the ground. We then pulled 20 mL of ambient air and vigorously shook the syringe for 2-3 minutes. After the shaking period, the gases in the headspace should be in equilibrium with the dissolved gas content. We transfered ~10-15 mL of the headspace air into a nylong, airtight syringe. The syringes were returned to the laboratory at the Toolik Field Station and were ran on a Shimadzu model 14A gas chromatograph with a thermal conductivity detector (TCD) and a flame ionization detector (FID) to determine CO2 and CH4 gas pressure, respectively. The sample chromatogram peak area was converted to concentration based on a standard curve (Kling et al. 2000).

2. Dissolved Inorganic Carbon (DIC) Sampling Description:

Using soil sampling needles and a 60 mL syringe, we pulled 20 mL of bubble-free water out of the ground. The water was transferred into a 20 mL serum bottles capped with aluminum capped teflon septa. We added 0.2 mL of Mercuric Chloride to preserve the sample. Samples were returned to the University of Michigan, the samples were acidified to convert all inorganic carbon to carbon dioxide, and the dissolved carbon dioxide was ran on a Shimadzu model 14A gas chromatograph using a TCD detector (Stainton 1973).

Kling, G. W., G. W. Kipphut, M. M. Miller, and W. J. O'Brien. 2000. Integration of lakes and streams in a landscape perspective: The importance of material processing on spatial patterns and temporal coherence. Freshwater Biol. (43): 477–497.

Shaver, G.R., and F.S. Chapin. 1991. Production - biomass relationships and elemental cycling in contrasting arctic vegetation types. Ecological Monographs 61(1): 1-31.

Stainton, M.P. 1973. A syringe gas-stripping procedure for gas-chromatographic determination of dissolved inorganic carbon and organic carbon in fresh water and carbonates in sediments. J. Fish Res. Bd. Canada 30:1441-1445.

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

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Variables Details

(Variables names are in the first line/row of the CSV file and Excel data sheet.)
Vegetation
  • Label: Vegetation
  • Definition: Vegetation Type: t = tussock tundra | w = wet sedge
  • Type: Nominal
  • Missing values: None specified
Treatment
  • Label: Treatment
  • Definition: Treatment Type: c = control | f = fertilized
  • Type: Nominal
  • Missing values: None specified
Plot#
  • Label: Plot#
  • Definition: Plot Number: 1 = plot 1 | 2 = plot 2 | 3 = plot 3
  • Type: Nominal
  • Missing values: None specified
Round
  • Label: Round
  • Definition: Replicate Identification: a = 1st replicate | b = 2nd replicate | c = 3rd replicate
  • Type: Nominal
  • Missing values: None specified
Location
  • Label: Location
  • Definition: Location sampled within the plot: for tussock, t = tussock | for tussock, n = inner tussock | for wet sedge, n = north | for wet sedge, s = south
  • Type: Nominal
  • Missing values: None specified
Date
  • Label: Date
  • Definition: Date sampled
  • Type: Date/time
  • Date format: dd-mmm-yy
  • Missing values:
    • . = Missing or Not Measured
Time
  • Label: Time
  • Definition: Time sampled
  • Type: Date/time
  • Date format: hh:mm
  • Missing values:
    • . = Missing or Not Measured
Category
  • Label: Category
  • Definition: Type of sample: d-dic = Dissolved Inorganic Carbon sample| d-gas = Dissolved gas sample
  • Type: Nominal
  • Missing values: None specified
Elev_m
  • Label: Elev_m
  • Definition: Elevation of the sample site
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
Depth_m
  • Label: Depth_m
  • Definition: Depth of the sample site
  • Type: Physical quantity
  • Unit: meter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
PCO2_uatm
  • Label: PCO2_uatm
  • Definition: Partial Pressure of Carbon Dioxide
  • Type: Physical quantity
  • Unit: microatmosphere
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
PCH4_uatm
  • Label: PCH4_uatm
  • Definition: Partial Pressure of Methane
  • Type: Physical quantity
  • Unit: microatmosphere
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
CO2_umol_L_gas_or_water
  • Label: CO2_umol_L_gas_or_water
  • Definition: Concentration of Carbon Dioxide per Liter of water or gas
  • Type: Physical quantity
  • Unit: micromolePerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
CH4_umol_L_gas_or_water
  • Label: CH4_umol_L_gas_or_water
  • Definition: Concentration of Methane per Liter of water or gas
  • Type: Physical quantity
  • Unit: micromolePerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured
DIC_umol_L
  • Label: DIC_umol_L
  • Definition: Concentration of Dissolved Inorganic Carbon
  • Type: Physical quantity
  • Unit: micromolePerLiter
  • Maximum: Not specified
  • Minimum: Not specified
  • Precision: Not specified
  • Missing values:
    • . = Missing or Not Measured