Percent carbon and percent nitrogen were measured from above ground plant and belowground stem biomass samples from experimental plots in moist acidic and moist non-acidic tundra. Biomass data are in 2001lgshttbm.dat.
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Plots at the acidic site (MAT) were set up in July 1996 on extra 5 x 20 meters plots within the four block design of the 1989 LTER acidic tussock experimental plots. On each plot a 5x10 meter section was fenced with large mesh (4-inch square mesh) and within this fence a 5x5-meter plot was fenced with a small mesh (1/2-inch square mesh). In each block two fenced plots were setup: a plot with no fertilizer and a plot with annual fertilization (NP) treatments of 10 g/m2 Nitrogen (as NH4NO3) and 5 g/m2 Phosphorous (as triple superphosphate). In this biomass harvest only the control unfenced (NFCT) and NP unfenced (NFNP) treatments were sampled. Plots at the non-acidic site (MNT) were setup in July 1997. Three replicate blocks were established with the following annual treatments in 5 x 20 m plots: control, nitrogen added (N), phosphorus added (P) and N and P (NP) in the same amounts as described for the MAT site. Two replicate blocks were established with a greenhouse treatment (GH) and a greenhouse fertilized treatment (GHNP). Greenhouses are annually set up in late May or early June and removed in the end of August or early September. In this biomass harvest only the control and NP treatments were sampled. Sampling Description. Biomass quadrats, size 20x20 cm, were taken from each site. Four quadrats were randomly located along line transects in each of the replicate blocks of each treatment. Aboveground biomass is considered "within" the quadrat if it is associated with a meristem that is within the quadrat. Quadrats were sorted within 24 hours into species and then into tissue type. The samples were dried at 50-70 degrees C in a drying oven and after several days weighed to nearest milligram. Details are given in Shaver and Chapin (Ecological Monographs, 61(1), 1991, pg. 1-31.) Samples were lumped by block and subsequently ground in a Wiley Mill and a Wig-L-Bug and shipped to the University of Nebraska, Lincoln to be analyzed for percent C and N on an elemental analyzer (ECS 4010, Costech Analytical, Valencia, California).
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