Biomass of soil rotifers, tardigrades, enchytraeids, protozoa and nematode groups from organic and mineral soils in moist acidic tundra nutrient addition plots (since 2006) sampled August 2012.
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In August 2012, soil samples were collected from control, and N+P plots from within a set of treatments in Moist Acidic Tundra plots established in 2006. At the time of sample collection we separated the soil into organic horizon, organic/mineral interface, and the upper 5cm of the mineral soil, and measured the depth of each layer. We determined percent moisture for all soils and quantified protozoa and nematode the biomass. Protozoa densities were estimated from 10 g soil samples using Most Probable Number MPN (Darbyshire et al. 1974; Ingham 1994). 10 g soil was serially diluted 10-1-10-6. Using a 24-well tissue culture plate, four replicate wells of each of the six dilutions were created by adding 0.5 ml soil solution to the wells (Rusterholz and Mallory 1994). E. coli, a bacterium food source for protozoa was added to each of the wells. Protozoa were separated into the broad categories of Amoebae, Flagellates and Ciliates. Nematodes were isolated from 20 g of soil samples using Baermann Funnels (Hall 1996). Nematodes were preserved in formalin, counted and identified to functional group based on the morphology of the stoma and stylet (Niles 1994). Rotifers, Tardigrades, and Enchytraeids were isolated from 5 g of soil using the methods of Peters et al. (1993).
Darbyshire, J. F., R. F. Wheatley, M. P. Greaves, and R. H. E. Inkson. 1974. A rapid micro method for estimating bacteria and protozoa in soils. Rev. Ecol. Biol. Sol. 11:465-475.
Hall, G.S. 1996. Methods for the examination of organismal diversity in soils and sediments. CAB International: New York.
Ingham, E.R. 1994. Protozoa. In: R.W. Weaver et al. (ed.), Methods of Soil Analysis. Part 2: Microbiological and Biochemical properties. Soil Science Society of America, Inc., USA. pp. 491-512.
Niles, R. 1994. Identification of nematode feeding groups. Unpublished manuscript.
Peters, U., Koste, W., and Westheide W. 1993. A quantitative method to extract moss-dwelling rotifers. Hydrobiologia 255-256: 330-341.
Data and metadata entered and updated R.Simpson Dec.2013, uploaded JD13Dec13
Version 2: Changed Distrubution URL since the LTER network DAS system is being discontinued. JimL 9Apr2015
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