In 1980-1982, six transplant gardens were established along a latitudinal gradient in interior Alaska from Eagle Creek, AK, in the south to Prudhoe Bay, AK, in the north (Shaver et al. 1986) .Three sites, Toolik Lake (TL), Sagwon (SAG), and Prudhoe Bay (PB) are north of the continental divide and the remaining three, Eagle Creek (EC), No Name Creek (NN), and Coldfoot (CF), are south of the continental divide. Each garden consisted of 10 individual tussocks transplanted back to their home-site, as well as 10 individuals from each of the other transplant sites.
Transplant gardens at Toolik Lake and Sagwon were established in 2014. At each location, 60 tussocks each from ecotypes of Eriophorum vaginatum from Coldfoot (CF, 67°15′32″N, 150°10′12″W), Toolik Lake (TL, 68°37′44″N, 149°35′0″W), and Sagwon (SAG, 69°25′26″N, 148°42′49″W) were transplanted. Half the transplanted tussocks were grown under ambient conditions, while the other half were exposed to passive warming supplied by open-top chambers (OTC).