Hyporheic exchange has the potential to significantly influence river temperatures in regions of continuous permafrost under low-flow conditions given the strong thermal gradients that exist in river bed sediments. However, there is limited understanding of the impacts of hyporheic exchange on Arctic river temperatures. To address this knowledge gap, heat fluxes associated with hyporheic exchange were estimated in a fourth-order Arctic river using field observations coupled with a river temperature model that accounts for hyporheic exchange influences. Temperature time series and tracer study solute breakthrough curves were measured in the main channel and river bed at multiple locations and depths to characterize hyporheic exchange and provide parameter bounds for model calibration. Model results for low-flow periods from 3 years indicated that hyporheic exchange contributed up to 27% of the total river energy balance, reduced the main channel diel temperature range by up to 1.7 degrees C, and reduced mean daily temperatures by up to 0.21 degrees C over a 13.1-km study reach. These influences are due to main channel heat loss during the day and gain at night via hyporheic exchange and heat loss from the hyporheic zone to the ground below via conduction. Main channel temperatures were found to be sensitive to simulated changes in ground temperatures due to changes in hyporheic exchange heat flux and deeper ground conduction. These results suggest that the moderating influence of hyporheic exchange could be reduced if ground temperatures warm in response to projected increases in permafrost thaw below rivers.