Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 2003, Arctic LTER, Toolik Research Station, Alaska.


Yearly file containing information on bacterial productivity. Samples were collected at various sites near Toolik Research Station (68 38'N, 149 36'W). Sample site descriptors include an assigned number (sortchem), site, date, time and depth.

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Published on EDI/LTER Data Portal


Kling, G. 2004. Bacterial Production Data for lakes and lake inlets/outlets samples collected summer 2003, Arctic LTER, Toolik Research Station, Alaska. Environmental Data Initiative.

Date Range: 

Wednesday, June 18, 2003 to Saturday, August 16, 2003

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For the bacterial production protocol, see the ARC LTER Land-Water group protocol accessible from the Land-Water mainpage on the Arctic LTER web page or at and clicking on the protocol link at the bottom of the page. Bacterial production is determined by the incorporation of 14C labeled Leucine (Simon and Azam 1989) in triplicate water samples incubated in the dark at in situ temperature for 1-3 hours (typically 2 hrs). Water samples are vacuum filtered through 0.22 um Millapore GS cellulose nitrate filters. Samples are extracted with 5mL of 5% TCA for 5 minutes, rinsed with additional TCA, and air dried in mini-scintillation vials. Following the addition of 1 mL cellosolve (ethylene glycol monoethyl ether), filters are left to dissolve overnight. Scinti-safe scintillation cocktail (4 mL) is then added to each vial and samples are counted at Toolik Field Station on a liquid scintillation counter (Packard Tri-Carb 2100TR) .Calculations of bacterial productivity assume that the Internal Dilution (ID) of leucine is 1, but studies show it may range from 1-2 (Kirchman 1993). Some published data from these LTER files have used an ID value different than 1; in those studies, the ID value used is stated in the article.1. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series 51:201-213.2. Kirchman, D.L. 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. Pages 509-512 in P.F. Kemp, B.F. Sherr, E.B. Sherr, and J.J. Cole, editors. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Florida, USA.

Version Changes: 

Entered metadata into new metadata form. ver2 corrected EML lat long Version 3: Data URL was changed to point to the LTERNET Data Access server Version 4: LTERNET Data Access server proxy changed from to Version 5: LTERNET Data Access server proxy changed from to Version 7: Update LTERNET Data Access server proxy link for Excel and comma delimited data files. Changed from knb to das in url. Version 8: Updated metadata form to newer version (without site sheet). Fixed discrepency with header variable names. CH 1Feb2013. Updated metadata to newer version (with sites sheet). CH March 2013. Updated time format, new URL, New eml for PASTA JD-Nov 2013 Version 9: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14 Moved to new web site and checked against file from Kling Lab. Jim L 15Mar16

Sites sampled.

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File 2003_bacprods_Kling.csv

Bacterial Production... File Details

File 2003_bacprods_Kling.xlsx

Excel file with... File Details