Number of cyanobacteria in Toolik Lake at 1 meter depth during June, July and August 1996 , Arctic LTER, summer 1996.

Abstract: 

Number of cyanobacteria in Toolik Lake at 1 meter depth during June, July and August 1996. Samples were transported to the Dept. of Fisheries and Oceans in West Vancouver, British Columbia, Canada for analysis.

Project Keywords: 

Data set ID: 

969

EML revision ID: 

5
Published on EDI/LTER Data Portal

Citation: 

Hobbie, J. 1998. Number of cyanobacteria in Toolik Lake at 1 meter depth during June, July and August 1996 , Arctic LTER, summer 1996. Environmental Data Initiative. http://dx.doi.org/10.6073/pasta/3ee39ca86220c42d24edb21238d62e2f
People

Owner/Creator: 

Contact: 

Additional People: 

Associated Researcher
Dates

Date Range: 

Wednesday, May 1, 1996 to Sunday, September 1, 1996

Publication Date: 

1998

Methods: 

Lake water was collected from the Toolik Lake main station at 1 m depth and preserved with 2%
glutaraldehyde. Samples were transported to the Dept. of Fisheries and Oceans in West Vancouver, British
Columbia, Canada for analysis. Twenty ml aliquots were filtered onto 0.2 black polycarbonate membrane
using a 25 mm filterholder, with vacuum pressure kept to less than 5 kPa. Filters were transferred to a
microscope slide and mounted with nonfluorescent immersion oil. Cells were counted at 1000x
magnification using a green-yellow (520-560 nm) excitation filter with an epifluorescence microscope.
Cell volumes were estimated by measuring the dimensions of subsets of cells with an eyepiece micrometer.
Calculations for cell volumes were done using formulae for geometric shapes approximating the shapes of
the cells. Synechococcus and Microcolonial Synechococcus assumed spheres with average cell
diamter 0.9 mm; Oscillatoria assumed cylinder with 0.8 mm width x average length.

Reference Citations: MacIsaac, E.A. and J.G. Stockner. 1993. Enumeration of phototrophic picoplankton by autofluorescence
microscopy. In Kemp, P., B.F. Sherr, E.B. Sherr and J.J. Cole (eds.) Handbook of Methods in Aquatic
Microbial Ecology, lewis Publishers, Boca Raton, pp. 187-197.

Version Changes: 

Version 3: Updated metadata sheet to newer version (with sites sheet). CH April 2013.
Version 5: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14
Changed Distribution URL since the LTER network DAS system is being discontinued. JimL 9Apr2015

Sites sampled.

Full Metadata and data files (either comma delimited (csv) or Excel) - Environmental Data Initiative repository.

Use of the data requires acceptance of the data use policy --> Arctic LTER Data Use Policy