The Changing Seasonality of Arctic Stream Systems (CSASN) was active from 2010 to 2012. The CSASN goal was to quantify the relative influences of through flow, lateral inputs, and hyporheic regeneration on the seasonal fluxes C, N, and P in an arctic river network, and to determine how these influences might shift under seasonal conditions that are likely to be substantially different in the future. There were a number of TASCC and Plateau nutrient additions at each sampling location. The data in this file contains nutrient concentrations from each sampling event during each tracer additions for spiraling curve characterization (TASCC )and Plateau nutrient addition during the CSASN project.
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Nutrient additions were conducted at various study streams. Most nutrient additions consisted on TASCC (Tracer Additions for Spiraling Curve Characterization). Please refer to Covino, T. , B. McGlynn, R. McNamara. 2010. Tracer Additions for Spiraling Curve Characterization (TASCC): Quantifying stream nutrient uptake kinetics from ambient to saturation. Limnol. Oceangr. 8:484-498. [doi: 10:4319/lom.2010.8.484]. Plateau additions also were conducted during the project, in which a particular nutrient were constantly added to the stream to stabilize a higher concentration of the nutrient for a certain amount of time. Throughout these additions, samples were collected in the field and filtered through a 25mm GF/F filter. Dissolved nutrients were analyzed with the filtered water. Many of the methods used for analyte analysis resembled the sampling protocol for LTER streams. The link to this protocol can be found below in the Sampling or Lab protocols section.
Ammonium was analyzed using the Salicylate method at the University of Vermont. Reference to this type of method can be found with the USGS. Method ID: I-2522-90.
Nitrate and Nitrate were analyzed using the cadmium reduction method to convert all nitrate to nitrite. Nitrite was then reacted with sulfanilamide and N-(1-naphthyl)-ethylenediamine dihydrochloride to form a colored azo dye. The formation of the color dye is proportional to the concentration of both nitrate and nitrite in the water sample. More detailed information about this method can be found on the EPA method for nitrate-nitrite: 353.4
Soluble Reactive Phosphorus:
Parsons et al. 1984. It involves the reaction of phosphorus with molybdate, ascorbic acid, and trivalent antimony. The molybdic acids are reduced to a blue-colored complex which is then read for absorbance on a UV spectrophotometer. A 1 cm cell is used in the spectrophotometer. Parsons, T.R., Maita, Y., Lalli, C.M., 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press, New York. pp. 173.
Conductivity was analyzed with a handheld instrument in the field.
Arctic LTER Streams Protocol, updated 2010 by EBS
2012 Data to be added soon.
Organized and Prepared by J. Benes: Nov. 2013
Version 2: Keywords added, file path updated, spelling corrected (JD-Dec2013)
Version 3: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 15Jan14