Organic soil from either the Anaktuvik severe burn or Toolik Lake were collected to test of effect of removal of mycorrhizae on decompositon of tundra at Toolik Lake and the Anaktuvuk Burn IN 2016.
A licor 6400 with 6400-09 soil respiration chamber was used to measure soil respiration (efflux) from the cores on a weekly basis.
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At Toolik Lake, between the reciprocal transplant gardens and the access road and at the Anaktuvik severe burn site 20 m east of the eddy flux town root and mycorrhizal exclusion experiments were established. 10 cm wide, 13cm long PVC collars had 4 x 5 cm holes cut into the side, 4 cm from the base. The holes and the base were wither covered with either a 2 mm plastic mesh or a ...um nylon mesh.
Organic soil from either the Anaktuvik severe burn or Toolik Lake was taken back to the lab, dried and put through a 4mm seive to remove large roots and stones. The cores were filled to 11 cm height with 138g of soil, resulting in a bulk density of 0.132 g/cm3.
Cores were established in the field on ... at Toolik and on ... at the burn. At each site, 5 blocks were established approximately 10 m apart. A block consisted of a patch of shrubs 9m2 in area. 3 cores were inserted into each shrub patch, 2 with the nylon mesh, one with the 2 mm plastic mesh. This was done by cutting a 8 cm wide hole to 11 cm depth so that the core fit very tightly with 2 cm visable above the soil. Approximately every 7 days one of the nylon cores (always the same one) was rotated by 30 ° in order to sever any in-grown mycorrhizal fungi, this was labeled with red tape and flagging in the field and called the 'H' treatment for 'heterotrophs only'. One of the nylon-mesh cores was left static to allow mycorrhizal ('MH' treatment) ingrowth and labeled with blue tape and flagging. The cores with 2 mm plastic mesh covering the holes allowed roots and mycorrhizae into the soil ('RMH' treatment) and labeled with yellow tape and flagging.
A licor 6400 with 6400-09 soil respiration chamber was used to measure soil respiration (efflux) from the cores on a weekly basis. The red 'rotated' cores were rotated AFTER the flux measurement.
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