Light-saturated photosynthetic rate, dark respiration, stomatal conductance and ratio of internal to external carbon dioxide concentration from the 1980-82 Eriophorum vaginatum reciprocal transplant plots from Eagle Creek to Prudhoe Bay, Alaska, 2010

Abstract: 

In 1980-1982, six transplant gardens were established along a latitudinal gradient in interior Alaska from Eagle Creek, AK, in the south to Prudhoe Bay, AK, in the north (Shaver et al. 1986) .Three sites, Toolik Lake (TL), Sagwon (SAG), and Prudhoe Bay (PB) are north of the continental divide and the remaining three, Eagle Creek (EC), No Name Creek (NN), and Coldfoot (CF), are south of the continental divide. Each garden consisted of 10 individual tussocks transplanted back to their home-site, as well as 10 individuals from each of the other transplant sites. Data were collected in July 2010 for tussocks transplanted in 1980-82 in a reciprocal transplant experiment and then harvested in 2011. Important variables are garden name, source population, light-saturated photosynthetic rate, dark respiration, stomatal conductance and ratio of internal to external carbon dioxide concentration.

Project Keywords: 

Data set ID: 

10501

EML revision ID: 

4
Published on EDI/LTER Data Portal

Citation: 

Fetcher, N., Mcgraw, J., Souther, S. 2013. Light-saturated photosynthetic rate, dark respiration, stomatal conductance and ratio of internal to external carbon dioxide concentration from the 1980-82 Eriophorum vaginatum reciprocal transplant plots from Eagle Creek to Prudhoe Bay, Alaska, 2010 Environmental Data Initiative. http://dx.doi.org/10.6073/pasta/ba7785eaad218efbe9c84b63805e2952
People

Owner/Creator: 

Contact: 

Additional People: 

Associated Researcher
Dates

Date Range: 

Monday, July 12, 2010 to Thursday, July 22, 2010

Publication Date: 

2013

Methods: 

I

In 1980-1982, six transplant gardens were established along a latitudinal gradient in interior Alaska from Eagle Creek, AK, in the south to Prudhoe Bay, AK, in the north (Shaver et al. 1986) .Three sites, Toolik Lake (TL), Sagwon (SAG), and Prudhoe Bay (PB) are north of the continental divide and the remaining three, Eagle Creek (EC), No Name Creek (NN), and Coldfoot (CF), are south of the continental divide. Each garden consisted of 10 individual tussocks transplanted back to their home-site, as well as 10 individuals from each of the other transplant sites (n = 10; 6 populations x 6 sites x 10 replicates = 360 total individuals).

Gas exchange was measured with an LI-6400 Portable Photosynthesis System (LiCor, Inc., Lincoln, NE, USA). Six leaves from each tussock were placed across a 2 x 3 cm cuvette that was equipped with an LED light source (6400-02B, LiCor, Inc., Lincoln, NE, USA). After measurement, width of each leaf was measured with a comparator. Leaf area was calculated as the sum of the leaf widths (cm) times the length of leaves that were in the cuvette (2 cm). Afterwards the portions of the leaves that were in the cuvette wre dried for 24 hr  at 65 C and weighed. For measurements of light saturated photosynthetic rate (AMAX), cuvette conditions were set to 380 ppm CO2, 15°C, 1500 μmol m-2 s-1 PPFD, and 40-70% relative humidity. After 10 measurements of AMAX, the light was switched off and 10 measurements of dark respiration (RESP) were made at 15°C after waiting 1 minute. The median

For AMAX and RESP measured on both leaf area and dry mass basis the 10 measurements were averaged to produce a mean value for each tussock. The median value of the 10 measurements wasc calculated. Mean and median values for the stomatal conductance and the mean ratio of internal  to external CO2 concentration were also calculated.

 Shaver GR, Fetcher N, Chapin FS (1986) Growth and flowering in Eriophorum vaginatum - Annual and latitudinal variation. Ecology 67:1524-1535

Notes:
Funding for this research was provided by National Science Foundation grant ARC-0908936 with additional support from NSF-BSR-9024188.
AMAX and RESP data are incorporated in a ms. submitted to Botany. Stomatal conductance and internal CO2 data are still unpublished.

Version Changes: 

Version 1: Metadata entered and data checked NF, data uploaded to Pasta (Jan2014-JD)
Version 2: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 24Jan14
Version 3: Checked keywords against the LTER network preferred list and replaced non-preferred terms. Jim L 27Jan14
Version 4: Removed abbreviations from title and abstract. Mar 2019 Jim L

Sites sampled.

Download a comma delimited (csv) or Excel file (includes metadata and data sheets).

Use of the data requires acceptance of the data use policy --> Arctic LTER Data Use Policy

To cite this data set see the citation example on the LTER Network Data Portal page for this data set.