Above ground plant biomass and leaf area were measured in a tussock tundra experimental site. The plots were set up in 1981 and have been harvested in previous years (See Shaver and Chapin Ecological Monographs, 61, 1991 pp.1-31).
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Plots were setup in June 1980 with annual fertilization treatments of 10 g/m2 Nitrogen (as NH4NO3) and 5 g/m2 Phosphorous (as triple superphosphate) were applied. Greenhouses and shade houses were annually set up in late May or early June and removed in the end of August or early September. Greenhouses and shade houses were discontinued in 1989. There are 4 replicate blocks, each containing 1 replicate of each of the following treatments. Control fertilized greenhouse greenhouse fertilized shade Notes: Cassiope was not separated into leaves and stems. Weights for Cassiope were recorded as leaf weights. Calculations: Units - Tissue mass data are expressed in g/quadrat (400 cm2). Multiply by 25 to get g/m2. Leaf blade areas are in cm2/quadrat. Multiply by .0025 to get m2 leaf/m2 ground. Sampling Description. Biomass quadrats, size 20x20 cm, were taken from Toolik tussock site. Five quadrats were taken from each of four blocks. All aboveground biomass were clipped in 20x20 cm quadrats. Five quadrats were randomly located along line transects in each of four replicate blocks in each treatment. Aboveground biomass is considered "within" the quadrat if it is associated with a meristem that is within the quadrat. Quadrats were sorted within 24 hours into species and then into tissue type. Depending on the harvest, tissue types can be broad categories, i.e. above and below, or more detailed, i.e. inflorescences, new growth, old growth, etc. Leaf area was measured with a LiCor LI-3000A area meter. Leaf areas for graminoids, deciduous, and forbs were measured immediately after plucking and before drying. Leaf areas for Evergreen species (except Ledum) were measured after drying for 2-4 days. Leaf areas for Ledum were measured after final dry weights were done. Cassiope leaf areas were measured on the unseparated leaves and stems. The samples were dried at 50-70 degrees C in a drying oven and after several days weighed to nearest milligram. Samples are then returned to Woods Hole for nutrient analysis. Details are given in Shaver and Chapin (Ecological Monographs, 61(1), 1991, pg. 1.)
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